Protein Info for GFF2218 in Sphingobium sp. HT1-2

Annotation: Thioredoxin reductase (EC 1.8.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 transmembrane" amino acids 111 to 129 (19 residues), see Phobius details PF00027: cNMP_binding" amino acids 33 to 115 (83 residues), 53.3 bits, see alignment E=6.8e-18 PF07992: Pyr_redox_2" amino acids 227 to 524 (298 residues), 115.3 bits, see alignment E=1.1e-36 PF13738: Pyr_redox_3" amino acids 319 to 507 (189 residues), 39.7 bits, see alignment E=1.1e-13 PF00070: Pyr_redox" amino acids 372 to 444 (73 residues), 31.3 bits, see alignment E=7.2e-11

Best Hits

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 75% identity to mdi:METDI5410)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>GFF2218 Thioredoxin reductase (EC 1.8.1.9) (Sphingobium sp. HT1-2)
METIGRDLREMQRVPLTAEHVAALRAEGSEAIYPAGTYIVRPGDPVDRFIYVEDGEIEVV
NPFGEERHVPSTIGPTQFMGEISFLGGGSWSIPMRAARDTRVLEVPRPAMLRLMAAIPEM
SDIIITVLAARRRRQLDMGDSTLVLIGEDEDRAVRCIAEFASRNRLPYTSHALGSDDARR
VAASCGAQADRPAVIFGRDNVVADPTPEKVARLFGLDRGFTENETVDLLIVGGGPAGVAA
AVYAGAEGLNALVVEDIAIGGQAGTSSRIENYMGFPTGISGADLVWRGEVQAMKFGTRFL
MPRRVTALEETGDGHYCGVFDNGQRICARAVLVATGVEYRRLPINRLADLEGAGIYYAAT
ENEARYCRQAEAVVVGGGNSAGQAAMFLSRSARHVHLLVRGPSLALSMSSYLSSRLEADP
AITIHYDTEIDSLHGDAQLDAVTIRHADGSAREIACCALFIMVGAAPNTGWLSGLVALDD
KGFVLTGDAFEAPSPYETSRAGIFAVGDVRAGSVKRVASAVGEGSVVISRIWSHVNDGSV
RPRVAASPAASPATG