Protein Info for GFF2195 in Variovorax sp. SCN45

Annotation: FIG00732904: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 89% identity to vpe:Varpa_5166)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.10

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1031 amino acids)

>GFF2195 FIG00732904: hypothetical protein (Variovorax sp. SCN45)
MMSFQDQLHACHERLVSVCAALPAPYPAFTLFFSVSDGSQRAHVVHARAADFETAWREGA
DSVGRWVRERGIVSPWLRIDWVEGASPMEWVQFKTQLTAVKRNYFRLGLAFDQDFELAFT
EQELNANAMLCGDSTIAHSVVNPRNFEVYGLARFERAPPLDMPPEQPVYLLAMVGVFCQP
DGVVHKLVGTGLDAGRRQLPALNPQSVLDLVRSGSSYLARQVQSDGLFVYGYFPCFDRRI
PTYDAMRHASAVYAMLEAWELTRDETLRAAIDRAVDHLTSWLIRDYTLADGRAAAFLVDT
GGEIKLGGNAACVLALVKYCELMDTRHWLPLIDKLALGIASLQDPATGRFNHVLHATDLT
VKQASRVIYYDGEAVYGLMRLYSLTRDPQWLAVVEKAFDYFIAGQHWQSHDHWLSCCVNE
LTHWSPQEKYFRFGLQNVAGYLDFVLDRKTTFPTLLELMLAAQQMLQRLEGMPAMRHLLD
ELDTEKFYRALDYRAHYLLNGFFWPEMAMYFRRPSTVVGSFFIRHHSFRVRIDDVEQYVS
GFAAYHRYLLERRSLAAANGTGGAPGNGARDDGPAWTASEMARVTGGEWVVRPEDGWRAS
GVTQRSFMRKGRVVFDHQTRPSKVPLAAVTLKGVLQPAAAVLCTDPKPHLDKRVPVLKVQ
NVLQSVLLLGDHARCEFTGRVCGVLGSGAGSGTVTAMLAGALTVWGEVAQPEGNISLPSG
IAWNMTCMPRHAAYWVMEMGASHMPASAQLVRPSLVVVTGLSAASQKHRGPSEAAARLDS
RIFQSMSPGDSVVLNRDMPEFATFAEAAMTQQLQIVTYGEHKDADVRLLAFDHGEVQALV
AGEPFQLRLNAPGRHMGASAIAALAALQALRLPLAAAVEPFAHFEPPGGRGALHTIHIGG
GSFKLIDETYDANPSSMRAALEMLSQAPCEPARRVAILGDMQELGPAAQRHHLDLEAELL
ASQPDRVLLCGPLMRALHTRIRTKVRSHWFVDVSDLSTALGGLLQPGDWVLAKSSAGIGL
SRLARVLKALP