Protein Info for GFF2190 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Glutamate synthase [NADPH] small chain (EC 1.4.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13247: Fer4_11
  • amino acids 54 to 145 (92 residues), 37 bits, E=2.3e-12
• PF12837: Fer4_6
  • amino acids 80 to 100 (21 residues), 27.2 bits, see alignment (E = 1.9e-09)
• PF00037: Fer4
  • amino acids 80 to 100 (21 residues), 27.1 bits, see alignment (E = 1.8e-09)
• PF12797: Fer4_2
  • amino acids 81 to 99 (19 residues), 24.7 bits, see alignment (E = 1.1e-08)
• TIGR01318: glutamate synthase, small subunit
  • amino acids 188 to 652 (465 residues), 844.1 bits, E=1.2e-258
• PF14691: Fer4_20
  • amino acids 205 to 315 (111 residues), 124.5 bits, E=1.3e-39
• PF07992: Pyr_redox_2
  • amino acids 305 to 408 (104 residues), 27.4 bits, E=1.6e-09
  • amino acids 329 to 640 (312 residues), 78.8 bits, E=3.5e-25
• PF00070: Pyr_redox
  • amino acids 329 to 363 (35 residues), 22.3 bits, see alignment (E = 1.1e-07)
• PF01494: FAD_binding_3
  • amino acids 329 to 360 (32 residues), 27.6 bits, see alignment (E = 1.2e-09)
• PF13450: NAD_binding_8
  • amino acids 332 to 366 (35 residues), 35 bits, see alignment (E = 1e-11)

Best Hits

Swiss-Prot: 85% identical to AEGA_ECOLI: Protein AegA (aegA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 87% identity to cro:ROD_24161)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (653 amino acids)

>GFF2190 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MNRFIMANSQQCLGCHACEVACVMAHNDERHVLTSQRYQPRITVIKHQHQRSAVTCHHCE
DAPCARSCPNGAIAHINDSVQVNAQKCIGCKSCVVACPFGTMQMVLTPVAPNQFKASAHK
CDLCQGREQGPACVENCPADALQLVTEDSLTRLAKTRRLRTARQEIRPWHTVDTQHSGAA
SSKAERMQATPPRGEPDKLAIEARKTTFEEIYLPFRAAQAEREASRCLTCGEHSICEWTC
PLHNHIPQWIELVKAGDIDAAVELSHQTNCLPEITGRVCPQDRLCEGACTLRDEYGAVTI
GNIERYISDRALSKGWRPDLSDVQKSDKRVAIIGAGPAGLACADVLARHGVSATVYDRHP
EIGGLLTFGIPAFKLDKSLLARRREIFSAMGIRFELNCEVGKDISLETLLESYDAVFVGV
GTYRSMKADLPNEDAPGVYDALPFLIANTKQVMGLPALPDEPFIDTAGLNVVVLGGGDTA
MDCVRTALRHGAANVTCAYRRDEANMPGSKKEVKNAREEGANFEFNVQPVELVLDTHGRA
SGIRFLRTRLGEPDGQGRRRPVPVPDSEFVMPADAVIMAFGFHPHGMSWLESHGVKVDNW
GRIAASVESEFRYQTSNPKIFAGGDAVRGADLVVTAMAEGRHAAQGILDWLAK