Protein Info for PS417_01110 in Pseudomonas simiae WCS417

Annotation: cysteine desulfhydrase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01275: pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family
  • amino acids 13 to 327 (315 residues), 360.2 bits, E=5.3e-112
• PF00291: PALP
  • amino acids 14 to 318 (305 residues), 170.2 bits, E=3.5e-54

Best Hits

Swiss-Prot: 66% identical to DCYD_YERPP: D-cysteine desulfhydrase (dcyD) from Yersinia pestis (strain Pestoides F)

KEGG orthology group: K05396, D-cysteine desulfhydrase [EC: 4.4.1.15] (inferred from 96% identity to pfs:PFLU0247)

MetaCyc: 61% identical to D-cysteine desulfhydrase (Escherichia coli K-12 substr. MG1655)
3-chloro-D-alanine dehydrochlorinase. [EC: 4.5.1.2]; D-cysteine desulfhydrase. [EC: 4.5.1.2, 4.4.1.15]

Predicted SEED Role

"D-cysteine desulfhydrase (EC 4.4.1.15)" (EC 4.4.1.15)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• glycine betaine degradation III (7/7 steps found)
• D-serine degradation (3/3 steps found)
• L-serine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• glycine betaine degradation I (6/8 steps found)
• L-cysteine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• glutathione-mediated detoxification I (3/8 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.15 or 4.5.1.2

Sequence Analysis Tools

See A0A1N7U3E0 at UniProt or InterPro

Protein Sequence (330 amino acids)

>PS417_01110 cysteine desulfhydrase (Pseudomonas simiae WCS417)
MIKQQLDRFNRLELLGGATALEKLERLSAWLGRDIYVKRDDTTPLAMGGNKLRKLEYLAA
DAIAQGADTLVTAGAIQSNHVRQTAALAAKLGLGCVALLENPTGTEDPNYLGNGNRLLLE
LFDAKVELVENLDNVDDQLNALADRLRNNGKKPYLVPIGGSNALGALGYVRAGLELAGQI
EDSGIEFAAVVLASGSAGTHSGLALALSEVLPNLPVIGVTVSRTDEAQRPKVAGLAERTA
ELLGVEIPEAFKVILWDEYFGPRYGEPNAGTLAAIKLLASQEGLLLDPVYTGKAMAGLLD
GIGRQRFEDGPIIFLHTGGAPALFAYDSAF