Protein Info for GFF2176 in Xanthobacter sp. DMC5
Annotation: L-lactate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to LLDD_XANP2: L-lactate dehydrogenase (lldD) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 85% identity to xau:Xaut_3929)MetaCyc: 76% identical to L-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
1.1.5.M6 [EC: 1.1.5.M6]; RXN0-7227 [EC: 1.1.5.M6]
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- L-lactaldehyde degradation (aerobic) (2/2 steps found)
- methylglyoxal degradation IV (2/3 steps found)
- methylglyoxal degradation V (2/3 steps found)
- superpathway of methylglyoxal degradation (5/8 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (3/8 steps found)
- superpathway of fucose and rhamnose degradation (4/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.2.3
Use Curated BLAST to search for 1.1.2.3 or 1.1.5.M6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (391 amino acids)
>GFF2176 L-lactate dehydrogenase (Xanthobacter sp. DMC5) VIISSASDYREAARRRLPPFLFHYIDGGAYAEVTLGRNVSDLTQLALRQRVLKSVGDVDL STTLLGEKLAMPMALAPVGLCGMFARRGEVQAAKAALDKGIQFTLSTVSVCPIEEVRAKV EKPFWFQLYVLKDRGFMKNALERAWAAGIRTLVFTVDMPVPGARYRDAHSGMSGPNAAVR RMVQAALHPFWAYDVGLMGTPHDLGNVSAYRGNKTTLEDYVGWLGANFDPSIGWRDLEWI REFWKGPMVIKGILDPEDAEDAVRFGADGIVVSNHGGRQLDGVLSSARALPPIADKVKGS LAILADSGIRSGLDVVRMVAQGADGVLLGRAFVYALATDGQKGVENLLDLFAKEMRVAMT LTGARNLSEISSESLVREVEQPLKNISAAAE