Protein Info for GFF2173 in Xanthobacter sp. DMC5

Annotation: Colistin resistance protein EmrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 537 transmembrane" amino acids 35 to 55 (21 residues), see Phobius details amino acids 74 to 94 (21 residues), see Phobius details amino acids 101 to 122 (22 residues), see Phobius details amino acids 129 to 152 (24 residues), see Phobius details amino acids 159 to 182 (24 residues), see Phobius details amino acids 188 to 211 (24 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 254 to 273 (20 residues), see Phobius details amino acids 293 to 313 (21 residues), see Phobius details amino acids 325 to 346 (22 residues), see Phobius details amino acids 357 to 376 (20 residues), see Phobius details amino acids 389 to 408 (20 residues), see Phobius details amino acids 423 to 442 (20 residues), see Phobius details amino acids 498 to 520 (23 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 34 to 521 (488 residues), 463.5 bits, see alignment E=4.2e-143 PF07690: MFS_1" amino acids 41 to 434 (394 residues), 153.2 bits, see alignment E=4.5e-49

Best Hits

Swiss-Prot: 43% identical to EMRB_ACIBT: Colistin resistance protein EmrB (emrB) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: None (inferred from 82% identity to xau:Xaut_1513)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (537 amino acids)

>GFF2173 Colistin resistance protein EmrB (Xanthobacter sp. DMC5)
MADTLTLKPSAAGPGAGSGGHAATPSAHVDGRRMFAFLCMVFGMFMAILDIQIVSASLAE
IQAGLAASSDEISWVQTSYLIAEVVMIPLSGFLSRALSTRWLFAASAAGFTIMSFMCATA
SSINEMIVYRALQGFLGGGMIPTVFAAAYSVFPREKQPIVAPMIGLVATLAPTIGPTVGG
YLTDLFSWHWLFLINVIPGMFVVLAAVTLIDFDEPDFSLLDKFDWWGLLFMAGFLGSLEF
VLEEGPTNDWFEDQYIVAFAIVCAVSSLAFFARVFSTDHPVVDLRAFENRNFTIGSAFSF
IMGIGLYGLTYLYPVFLGRVRGYSALQIGETMFVTGIAMFLTAPIAGRLMTKIDPRIMMG
AGFAAFAVGTWWVTFITKDWDFWELFVPQILRGVGLMIAMIPINNISLGTMPPAQMKNAS
GLFNLMRNLGGAVGLALINTVLNNRWDLHIARLHEHVEWASATAVERLDTITAGFAKLGS
DASSAALKQMAAIVRQQALVMAFADVFYLLTFLFFALVIASPLMRRPKPGAGGGGGH