Protein Info for GFF2160 in Variovorax sp. SCN45

Annotation: Aspartate carbamoyltransferase (EC 2.1.3.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02729: OTCace_N
  • amino acids 17 to 160 (144 residues), 141.1 bits, E=3e-45
• TIGR00670: aspartate carbamoyltransferase
  • amino acids 17 to 315 (299 residues), 305.7 bits, E=1.6e-95
• PF00185: OTCace
  • amino acids 168 to 314 (147 residues), 95.3 bits, E=4.2e-31

Best Hits

Swiss-Prot: 98% identical to PYRB_VARPS: Aspartate carbamoyltransferase (pyrB) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00609, aspartate carbamoyltransferase catalytic subunit [EC: 2.1.3.2] (inferred from 98% identity to vpe:Varpa_5218)

Predicted SEED Role

"Aspartate carbamoyltransferase (EC 2.1.3.2)" in subsystem De Novo Pyrimidine Synthesis (EC 2.1.3.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• UMP biosynthesis I (5/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.2

Use Curated BLAST to search for 2.1.3.2

Protein Sequence (320 amino acids)

>GFF2160 Aspartate carbamoyltransferase (EC 2.1.3.2) (Variovorax sp. SCN45)
MLYKRNPQLNKHGELIHLLSIEGLPKDIVTHILDTAANFTSVSDREVKKVPLLRGKSVFN
LFFENSTRTRTTFEIAAKRLSADVINLDIARSSATKGESLLDTIANLSAMAADLFVVRHS
ESGAPYLIAQHVAPHVHVVNAGDGRHAHPTQGLLDMYTIRHYKKDFSNLTVAIVGDVLHS
RVARSDIHALTTLGCAEVRVVGPKTLVPADMAGMGVRVFHTLEEGIKDCDVVIMLRLQNE
RMSGALLPSSQEFFKTFGLTPEKLQLAKPDAIVMHPGPINRGVEIDSAVVDGKQSVILPQ
VTFGIAVRMAVMSIVAGNEA