Protein Info for PS417_11015 in Pseudomonas simiae WCS417
Updated annotation (from data): Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26)
Rationale: Specifically important for: L-Arabinose. alpha-ketoglutarate semialdehyde is an intermediate in the oxidation of L-arabinose (SEED_correct)
Original annotation: ketoglutarate semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K14519, NADP-dependent aldehyde dehydrogenase [EC: 1.2.1.4] (inferred from 95% identity to pfs:PFLU2370)MetaCyc: 77% identical to 2-ketoglutarate semialdehyde dehydrogenase (Pseudomonas aeruginosa PAO1)
2,5-dioxovalerate dehydrogenase. [EC: 1.2.1.26]
Predicted SEED Role
"Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Proline, 4-hydroxyproline uptake and utilization (EC 1.2.1.26)
MetaCyc Pathways
- superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
- trans-4-hydroxy-L-proline degradation II (4/4 steps found)
- D-galactarate degradation II (3/3 steps found)
- D-glucarate degradation II (3/3 steps found)
- L-arabinose degradation III (5/6 steps found)
- D-glucuronate degradation II (4/5 steps found)
- cytosolic NADPH production (yeast) (4/5 steps found)
- mitochondrial NADPH production (yeast) (4/5 steps found)
- 1,2-dichloroethane degradation (3/4 steps found)
- L-lyxonate degradation (2/3 steps found)
- pyruvate fermentation to acetate VIII (1/2 steps found)
- D-galacturonate degradation II (3/5 steps found)
- L-carnitine degradation II (1/3 steps found)
- D-arabinose degradation III (2/6 steps found)
- D-xylose degradation III (1/5 steps found)
- D-xylose degradation V (1/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)
- superpathway of pentose and pentitol degradation (16/42 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.26, 1.2.1.4
Use Curated BLAST to search for 1.2.1.26 or 1.2.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UGH9 at UniProt or InterPro
Protein Sequence (526 amino acids)
>PS417_11015 Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (Pseudomonas simiae WCS417) MTSFLGHNYIGGQRSANGSVTLQSVDATSGEALPQHFYQATPQEVDAAAKAAAQAYPAYR ALSAARRAQFLDAVADELDALGDEFVELVCRETALPAARIKGERGRTSGQMRLFATVLRR GDFYGARIDKALPDRQPMPRPDLRQYRIGLGPVAVFGASNFPLAFSTAGGDTAAALAAGC PVVFKAHSGHMATAERVADAIIRAAEATEMPAGVFNMIFGGGVGEALVKHPAIQAVGFTG SLKGGRALCDMAAARPQPIPVFAEMSSINPVIVLPQALKARAESVARDLTASVVQGCGQF CTNPGLVIGVASPEFTAFTQQVAQLIGDQPAQTMLNAGTLSSYGKGLEKLLAHPGIQHLA GSQQAGNQAQPQLFKADVRLLIDGDEVLQEEVFGPTTVFVEVADQAQLSAALHGLHGQLT ATIIGEPADLQQFAELTPLLEQKVGRILLNGYPTGVEVCDSMVHGGPYPATSDARGTSVG TLAIDRFLRPVCFQNYPDSLLPDALKNANPLRIQRLVDGTPSRDPL