Protein Info for GFF2150 in Xanthobacter sp. DMC5

Annotation: Potassium-transporting ATPase ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 767 transmembrane" amino acids 157 to 177 (21 residues), see Phobius details amino acids 182 to 227 (46 residues), see Phobius details amino acids 231 to 249 (19 residues), see Phobius details amino acids 381 to 401 (21 residues), see Phobius details amino acids 413 to 436 (24 residues), see Phobius details amino acids 719 to 737 (19 residues), see Phobius details amino acids 743 to 762 (20 residues), see Phobius details PF00403: HMA" amino acids 10 to 68 (59 residues), 50.5 bits, see alignment 3.4e-17 TIGR01512: cadmium-translocating P-type ATPase" amino acids 211 to 527 (317 residues), 322.4 bits, see alignment E=1e-99 TIGR01525: heavy metal translocating P-type ATPase" amino acids 211 to 761 (551 residues), 582.8 bits, see alignment E=1.7e-178 TIGR01511: copper-translocating P-type ATPase" amino acids 229 to 761 (533 residues), 410.7 bits, see alignment E=2.3e-126 TIGR01494: HAD ATPase, P-type, family IC" amino acids 234 to 737 (504 residues), 270.3 bits, see alignment E=4.9e-84 PF00122: E1-E2_ATPase" amino acids 262 to 442 (181 residues), 182 bits, see alignment E=1.2e-57 PF00702: Hydrolase" amino acids 459 to 673 (215 residues), 112.8 bits, see alignment E=4.2e-36

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 76% identity to xau:Xaut_3338)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (767 amino acids)

>GFF2150 Potassium-transporting ATPase ATP-binding subunit (Xanthobacter sp. DMC5)
MTAGQVQTRYRVSGMDCASCAAKIEAVASRLDGVAEASVSVAAATLVVRHVSDADLSVLP
ARLKPMGYGLVLANAEPVRTTAPVSVREACCGHDHGADLGGHDHGSHDHTDHHHAGEDHA
GHDHSGHPDRTAPAVGHEHAHAAETGEGGFWPNGKLRLTLASGAAFAAAFLAGLVLPQVQ
TPLFIAAMLVGLVPIARRAFAAAAVGMPFTIETLMTIAAVGAVIIGAAEEAAAVVFLFLV
GELLEGFAAGRARDGIKALAALVPKTALVARGGDFVEVPAAQLAMGDTILVRPGDRIAAD
GEVVAGEGGIDESMVTGESAPVTKGVGANVFAGTVNGDAALTVRVTATAEDNTIARVVKL
VEEAQEARAPTERLIDRFSRVYTPAVVVIGALVATVPPLLLGGDWGEWVYKGLAILLIGC
PCALVISTPAAIASGLSAGARRGLLLKGGAVLETAGKVTVVCLDKTGTLTEGRPKVTDVI
GLALPEPEVLRLAAALEGGSSHPLAKAILERAAAEGLAVPAASDGRALGGKGVTGLVEGR
DVFLGSVAAGAARAALDRDLEARLAALNDEGKTVSLLIVDDALAGAIAMRDEPRADAAAG
LAALKTRGLRTLMLTGDNARTAAAIGRVLGIEVRAGLLPEDKQRIVSELKRQGDIVAKVG
DGINDAPALAAADIGIAMGGGTDVALETADAAVLHGRVRDIASMIHLSRRTMRNIRQNIG
ISLGLKAVFLVTTVAGITGLWPAILADTGATVLVTANALRLLRRTEG