Protein Info for GFF2149 in Sphingobium sp. HT1-2

Annotation: Thymidylate synthase (EC 2.1.1.45)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to TYSY_SPHAL: Thymidylate synthase (thyA) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K00560, thymidylate synthase [EC: 2.1.1.45] (inferred from 89% identity to sch:Sphch_1206)

Predicted SEED Role

"Thymidylate synthase (EC 2.1.1.45)" in subsystem Folate Biosynthesis (EC 2.1.1.45)

MetaCyc Pathways

superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
folate transformations III (E. coli) (8/9 steps found)
superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
dTMP de novo biosynthesis (mitochondrial) (3/3 steps found)
pyrimidine deoxyribonucleosides salvage (4/5 steps found)
pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
folate transformations II (plants) (8/11 steps found)
pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.45

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (314 amino acids)

>GFF2149 Thymidylate synthase (EC 2.1.1.45) (Sphingobium sp. HT1-2)
LHAMSPQPPHYEEQYLDLMRHIWRHGDERIDRTGVGTRSILGATLRFSLADDAVPLLTTK
RVYWKVAAREMLWFLTGDTNIRELVKQNVHIWTDWPLDSYRKATGEDIDRDAFEARIIED
EAFARQWGDLGPVYGAQWVNWPRYEPAGDGLFRRAAQGHNQIAALVDAIRTNPGSRRLLF
TGWNVAEVAQMALPPCHMTYQFQVSDGRLNGLLFQRSCDLGLGFAFNIFGLSMITRMLAQ
QCDLEPGEVVWQGGDVHLYLNHADLVEEQISRTPAGAPKLRINRRPSSIFDYGIEDFEVQ
DYAPQAHISAPVAV