Protein Info for GFF2144 in Xanthobacter sp. DMC5
Annotation: Nitrilase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to NITR2_BRADU: Nitrilase blr3397 (blr3397) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
KEGG orthology group: None (inferred from 82% identity to rpx:Rpdx1_4378)MetaCyc: 51% identical to Nit2 (Arabidopsis thaliana col)
Nitrile hydratase. [EC: 4.2.1.84]; Nitrilase. [EC: 4.2.1.84, 3.5.5.1]; 3.5.5.1 [EC: 4.2.1.84, 3.5.5.1]
Predicted SEED Role
"Plant-induced nitrilase (EC 3.5.5.1), hydrolyses beta-cyano-L-alanine" (EC 3.5.5.1)
MetaCyc Pathways
- superpathway of acrylonitrile degradation (3/3 steps found)
- acrylonitrile degradation I (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- indole-3-acetate biosynthesis V (bacteria and fungi) (1/1 steps found)
- aldoxime degradation (2/3 steps found)
- indole glucosinolate activation (herbivore attack) (8/13 steps found)
- indole-3-acetate biosynthesis II (6/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via CoA ligation
- Cyanoamino acid metabolism
- Nitrogen metabolism
- Styrene degradation
- Tryptophan metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.5.1, 4.2.1.84
Use Curated BLAST to search for 3.5.5.1 or 4.2.1.84
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (310 amino acids)
>GFF2144 Nitrilase (Xanthobacter sp. DMC5) MPRIKVAAVQAATIPFDGSAATQRTVALIAEAATTGAKIAVFPEAFIGGYPKGLDFGCSV GRRTAEGRADFVRYVRGAITVPGPEVDQLAAACAEHDIHVVVGVIERDGGTLHCTVLYLG PDGLLGKHRKVMPTGSERLVWGFGDGSTLTVVDTPYGKLGGAICWEHYMPLLRAAYYGKG VEIWAAPTADDRETWISTMRHVAMEGRCFVISACQVMRRSDFPEDYASRIEAAPDAWMMH GRSVIVGPLGEVLAGPVLDEETILTAEIDLDDIIGAKLDFDPIGHYARPDLFSLNVDEAP QLPVVARGEG