Protein Info for GFF2108 in Sphingobium sp. HT1-2

Annotation: NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 34 to 51 (18 residues), see Phobius details amino acids 58 to 77 (20 residues), see Phobius details amino acids 82 to 102 (21 residues), see Phobius details amino acids 114 to 132 (19 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 177 to 197 (21 residues), see Phobius details amino acids 213 to 236 (24 residues), see Phobius details amino acids 254 to 276 (23 residues), see Phobius details amino acids 283 to 305 (23 residues), see Phobius details amino acids 312 to 336 (25 residues), see Phobius details amino acids 340 to 360 (21 residues), see Phobius details amino acids 381 to 406 (26 residues), see Phobius details amino acids 426 to 448 (23 residues), see Phobius details amino acids 516 to 536 (21 residues), see Phobius details amino acids 570 to 590 (21 residues), see Phobius details amino acids 671 to 691 (21 residues), see Phobius details TIGR01974: proton-translocating NADH-quinone oxidoreductase, chain L" amino acids 1 to 690 (690 residues), 856.9 bits, see alignment E=6.6e-262 PF00662: Proton_antipo_N" amino acids 65 to 115 (51 residues), 72.9 bits, see alignment 2.3e-24 PF00361: Proton_antipo_M" amino acids 131 to 422 (292 residues), 291.1 bits, see alignment E=1.3e-90 PF06455: NADH5_C" amino acids 567 to 683 (117 residues), 42 bits, see alignment E=1.2e-14

Best Hits

KEGG orthology group: K00341, NADH dehydrogenase I subunit L [EC: 1.6.5.3] (inferred from 90% identity to sjp:SJA_C1-13340)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (693 amino acids)

>GFF2108 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) (Sphingobium sp. HT1-2)
MIQLIVLLPLLAAAIAGLGNKALGKIPAKIVTTGALFAACAMSWPIFISFLTGHAEPYVA
PVFTWIESGSFDAQWALRVDTMTAVMLVVITSVSSLVHLYSWGYMDEEPDQPRFFAYLSL
FTFAMLMLVTANNLLQMFFGWEGVGLASYLLIGFWFRKPSANAAAIKAFVVNRVGDLGFM
MGIFGTYLVFNTISIPEILEMAPSMAGSTIGFLGHRFDTMTVLCLLLFIGAMGKSAQLGL
HTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRLSPMFEMSPTALTVVTYVGAATCLFAA
TVGTVQTDIKRVIAYSTCSQLGYMFFAAGCGAYGAAMFHLFTHAFFKALLFLGAGSVIHA
MHHEQDMRYYGGLRKKIPVTFWTMTLGTLAITGVGLPLVGVGFAGFYSKDGILEAAFASG
GAGIGAYYVGVFAALLTSFYSWRLVFLTFFGKPRWTQSEHIQHALHDDHGHGHDDHAHVG
HGHDDHSHAHADHGHDHHAHDAGDGTGGYHPHESGLVMLIPLLVLSLGAVFAGFVFHDQF
IGPEGGIEFWKGALAFDSHLMHAAHEVPTWVKFGPFTVMLTGLVIAWLAYIKNTDWPQRF
VATFGALHQFLLNKWYFDELYNFLFVKPAFAIGRFFWQFGDVGFIDRFGPNGLAALVVQG
NKITRRLQSGYLYTYALVMLIGLAAAATWAMTR