Protein Info for GFF2106 in Sphingobium sp. HT1-2

Annotation: NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 8 to 462 (455 residues), 453.5 bits, E=5.1e-140
• PF00361: Proton_antipo_M
  • amino acids 123 to 416 (294 residues), 270.5 bits, E=8.4e-85

Best Hits

Swiss-Prot: 68% identical to NUON_SPHWW: NADH-quinone oxidoreductase subunit N (nuoN) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 86% identity to sjp:SJA_C1-13360)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Protein Sequence (478 amino acids)

>GFF2106 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) (Sphingobium sp. HT1-2)
MIETASLLAVLPELILTGSGLVLMLIAAFGGDRSTHAVNWLAVLALLAAGLSLCTSMAHG
PVAFDGLVRADAFSVYAKALIYGAAGAAILLAPRFFATGGALRPEYAVLIVFAAIGMGMM
VSAGDMLTLYVGLEMNSLASYVLASFMRQDEKSSEAGLKYFVLGSLASGILLYGISLLYG
FTGSTAFDGIAIAMGDGVSKGELFGLVFVLAGLAFKISAVPFHMWTPDVYEGAPTPVTAF
FGSAPKVAAMGLMVRVAIEALGPAGLDWQQIIIFVALASILFGAVAAIGQTSIKRLMAYS
SINNVGFALVGLAAGTPAGVAATMSYMAIYVVMTIGAFACILQMRDADGKPVDTIASLAG
LSQSRKGLSAAFAIFMFSMAGIPPLFGFWAKFLVFDAAVAANLTALAAFGIAMSVIGAFY
YLKIIKTIYFDEPAAPYEAKGGAVENIIMAACAIVIVFGYLLNPLLDKASAAAAASLF