Protein Info for PS417_10700 in Pseudomonas simiae WCS417

Annotation: NAD(P)H dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 transmembrane" amino acids 89 to 109 (21 residues), see Phobius details amino acids 175 to 187 (13 residues), see Phobius details PF03358: FMN_red" amino acids 1 to 151 (151 residues), 42.2 bits, see alignment E=6.4e-15 PF02525: Flavodoxin_2" amino acids 1 to 208 (208 residues), 168.4 bits, see alignment E=1.7e-53

Best Hits

Swiss-Prot: 42% identical to NQO2_PONAB: Ribosyldihydronicotinamide dehydrogenase [quinone] (NQO2) from Pongo abelii

KEGG orthology group: K00355, NAD(P)H dehydrogenase (quinone) [EC: 1.6.5.2] (inferred from 86% identity to pfl:PFL_2716)

MetaCyc: 38% identical to NAD(P)H dehydrogenase (quinone) 1 (Homo sapiens)
NAD(P)H dehydrogenase (quinone). [EC: 1.6.5.2]; 1.6.5.2 [EC: 1.6.5.2]

Predicted SEED Role

"NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2" (EC 1.6.99.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.2, 1.6.99.-

Use Curated BLAST to search for 1.6.5.2 or 1.6.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1SNF9 at UniProt or InterPro

Protein Sequence (233 amino acids)

>PS417_10700 NAD(P)H dehydrogenase (Pseudomonas simiae WCS417)
MKVLIVHAHPEPQSFTAALRDRAVATLEGLGHAVKVSDLYAMRWNPVASAADFSSRENPD
YLVYALEQRLGVKKQSIAADIQGELDKLLWADLLILNFPIFWFSAPAMLKGWIDRVLVSG
VCYGGKRFYDQGGLAGKRAMITVTLGGREHMFGEGAIHGPLEDMLRPILRGTLAYVGYDV
LAPFVAWHVPYISAEARQDFLVAYEQRLQGLADEQPLVFPKLAQFDEALYPLS