Protein Info for GFF2087 in Sphingobium sp. HT1-2

Annotation: Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1086 (1085 residues), 1500.4 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 234.5 bits, E=3.8e-73
  • amino acids 709 to 910 (202 residues), 117.2 bits, E=3.2e-37
• PF02787: CPSase_L_D3
  • amino acids 425 to 502 (78 residues), 89.3 bits, 5.3e-29
• PF02222: ATP-grasp
  • amino acids 717 to 881 (165 residues), 28.1 bits, E=6.1e-10
• PF07478: Dala_Dala_lig_C
  • amino acids 734 to 879 (146 residues), 30 bits, E=1.5e-10
• PF02655: ATP-grasp_3
  • amino acids 738 to 881 (144 residues), 27.1 bits, E=1.6e-09
• PF02142: MGS
  • amino acids 992 to 1077 (86 residues), 65.5 bits, 1.6e-21

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1111 amino acids)

>GFF2087 Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) (Sphingobium sp. HT1-2)
MPKRTDISSILIIGAGPIIIGQACEFDYSGTQAVKALKEEGYRIILVNSNPATIMTDPEF
ADATYVEPITPEIVAKIIEKERPDAVLPTMGGQTALNTALALFNDGTLEKFGVQMIGADA
EAIDKAEDRIKFRDAMDKIGLESARSRIAHTMEEAMEALEFTGLPSIIRPSFTMGGTGGG
IAYNRDEFMNIVRGGLDASPTTEVLIEESLLGWKEYEMEVVRDRNDNSIIICSIENVDPM
GVHTGDSITVAPALTLTDKEYQIMRNASLAVLREIGVETGGSNVQFAVNPKDGRLVVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEIENDITGATPASFEPTIDYVVTKIPR
FAFEKFKGAEPLLGTAMKSVGEVMAIGRNIHESMQKALRGLETGLSGFNQVDHLVGAPKD
DIIAALAQPTPDRLLVAAQALREGLTVAEIHNIAKFDPWFLERLKEIVEAENEVLEHGLP
RDADGMRRLKAMGFSDKRLAWLALKSANLRGNERGVARGSGLIHDAVVAMTGGVTEDEVR
SLRHKLGVRPVFKRIDTCAAEFEAKTPYMYSTYEAPIFGEPENEAQPSDRKKVVILGGGP
NRIGQGIEFDYCCVHACFALSEAGYETIMVNCNPETVSTDYDTSDRLYFEPLTAEDVLEI
LHVEMSKGTLAGVLVQFGGQTPLKLAQALEDAGIPILGTSPDAIDLAEDRERFAALIDKL
KLRQPANGIARSREEAIAVANRIGYPVLMRPSYVLGGRAMEIVDGQAQLEEYITTAVQVS
GDSPVLIDQYLRDAVEVDVDALCDGDDVVVAGVLQHIEEAGVHSGDSACSLPPYSLSDEV
IAEIERQTEVLARALSVRGLMNIQFAVKDGIVYLIEVNPRASRTVPFVAKAIGIPIAKIA
SRVMAGEKIKDLPKIDRNAIDHVAVKEAVFPFNRFPGVDPVLSPEMKSTGEVMGIDSNFA
TAFAKAQLGAGTVLPTSGTVFVSVKDSDKPVILPAVQKLAGMGFTIIATGGTATYLEGQG
IAVEHVNKVAEGRPHIVDRITDGDVQLIFNTTEGWQSLKDSKAIRTSALRAKIASFTTAT
ASVAAADAIEALRDRALEVRSLQSYYPGSHA