Protein Info for GFF2080 in Variovorax sp. SCN45

Annotation: Protein acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 transmembrane" amino acids 388 to 406 (19 residues), see Phobius details PF13380: CoA_binding_2" amino acids 18 to 144 (127 residues), 75.9 bits, see alignment E=7.5e-25 PF02629: CoA_binding" amino acids 19 to 108 (90 residues), 43.1 bits, see alignment E=1.2e-14 PF13607: Succ_CoA_lig" amino acids 164 to 300 (137 residues), 163.5 bits, see alignment E=4.9e-52 PF13549: ATP-grasp_5" amino acids 484 to 698 (215 residues), 207.8 bits, see alignment E=2.8e-65

Best Hits

KEGG orthology group: None (inferred from 71% identity to del:DelCs14_5133)

MetaCyc: 42% identical to pimeloyl-CoA synthetase monomer (Pseudomonas mendocina 35)
6-carboxyhexanoate--CoA ligase. [EC: 6.2.1.14]

Predicted SEED Role

"Protein acetyltransferase" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>GFF2080 Protein acetyltransferase (Variovorax sp. SCN45)
MDFFSSQAESLSALFAPRAIAVVGASSNPQKIGGIPVDYQRRFGFDGALYPVNPNADRIQ
ELQAWPSLRAIGQPVDLAILAVPAALVDSALDDAIAAKVKGVVLFSSGFAEIGAEGTAAQ
ARLGDKARAAGVRLIGPNCLGFMNIARNVYATFSPAPGVGRVTAGRIGLVSQSGAFGAYA
YSMARARGVGLSLWATTGNEADVQFADCLAWLAQDPATDVIMGYMEGCRDGPRLRAALAL
AQANGKPVVMVKVGSTALGAQAAASHTAALAGDDAVYDAVFRQYGVLRARNLTEFFDLAH
SAAVAGRPRDRSIGLFTLSGGVGALMADEASAQGLDVQPLSDAAQDTLRGWVPFAAPRNP
VDITGQVTNDMSLLERSARVMLDDRGFASWMGFLAAAGASDAFWPVLRELVGSLRKAYPD
TLLAISTLLAPERRAELEAMRCLVFADPSDGIRTIAALAGLKTGAARSADSTPPSNPLKL
APGTMSEPDALSLLAEAGVPVVEHRVVRSADEAAAAAEAIGDAVVVKIVSADIPHKSDVG
GVALGLRGAAQARAAFERTRDHALTARPDARLDGALVARMLGGGVECIAGVHRDPVFGPV
LMFGLGGIHVETLRDVSLRALPIARDDALAMVRELRAFAILDGARGRAPVDLESIADALC
ALAGFALRAGDSLDSAEINPLIARPLADGGCVAVDALVVGRGE