Protein Info for GFF2076 in Variovorax sp. SCN45

Annotation: Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 PF00753: Lactamase_B" amino acids 34 to 228 (195 residues), 33.6 bits, see alignment E=3.9e-12 PF14863: Alkyl_sulf_dimr" amino acids 277 to 415 (139 residues), 111.3 bits, see alignment E=5.2e-36

Best Hits

KEGG orthology group: None (inferred from 79% identity to del:DelCs14_5137)

Predicted SEED Role

"alkyl sulfatase( EC:3.1.6.- )"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (423 amino acids)

>GFF2076 Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily (Variovorax sp. SCN45)
MSTNDSTNTVEQEKPFLIVGTGTETVAPGLHILRGQGQSFVAETDAGLVVIDAGPGGPVT
QGMIDAVRQLSNAPLHALCYSHGHVGYNSGVPMWLEHAAQRGDAPPRVIAHRNVLRRQAR
YRETETLQHRMAEIQFNRPAGFFDRRLAMHAPTETFDDRLQIGQGDSHIELRWAPSETDD
AIALWSPSQRVLYGGAALIDSIPNIGTPFRTMRDTVRWADTLDALAALGARKAVREFGAT
LEGEEQVRHVLTHTARALRWLRAEVVRLMNEGLNERGILARIRFPDELFGVEWMKPTYGD
PGYIVRDIYRSENGWWDRNPTSLHPEAPEAIGRAVADAISDKRGVITHARSLAQGGQWQL
ALHVIDLLATADGDAPEIAEARSLKAEWLRERARQVPSYVSRNLYRVGADMIEQGTQARF
GIH