Protein Info for GFF2070 in Sphingobium sp. HT1-2

Annotation: Methylenetetrahydrofolate--tRNA-(uracil-5-)- methyltransferase TrmFO (EC 2.1.1.74)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR00137: tRNA:m(5)U-54 methyltransferase" amino acids 5 to 441 (437 residues), 555.1 bits, see alignment E=5.3e-171 PF01134: GIDA" amino acids 6 to 377 (372 residues), 323.4 bits, see alignment E=2.2e-100

Best Hits

Swiss-Prot: 76% identical to TRMFO_SPHAL: Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO (trmFO) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K04094, glucose inhibited division protein Gid (inferred from 90% identity to sjp:SJA_C1-14560)

Predicted SEED Role

"tRNA:m(5)U-54 MTase gid" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF2070 Methylenetetrahydrofolate--tRNA-(uracil-5-)- methyltransferase TrmFO (EC 2.1.1.74) (Sphingobium sp. HT1-2)
MSNHQIHVIGGGLAGSEAAWQLAQAGIKVRLSEMRGTGEMTPAHQTDGLAELVCSNSFRS
DDADKNAVGLLHQEMRRLGSLIMDRAEPAKVPAGSALAVDRHVFSDGVTRALAEHPNVEI
VRERIDTLPTEGMTIVATGPLTASALASSIGQAAGMEHLAFFDAIAPVIHFDSIDMEECW
MANRWDKIGPGGGEGKDYINCPMNKEQYQAFVQGLLDGEKTEFKEWESSTPYFEGCMPIE
VMASRGPETLRHGPMKPMGLDNPKTGRWPYAVVQLRQDNASGTLWNMVGFQTKLKHGAQV
ELFRTIPGLEKAEFARLGGLHRNTFIQSPKLLDPTLRLKSAPHIRFAGQMTGCEGYVESA
AIGLIAGRFAAAEILGQELAPPPADTALGALLGHVTGNVETADYQPMNVNFGLFPTLDDV
KKKQRKEAYTARARASFGRWLGEVVEPA