Protein Info for GFF2061 in Methylophilus sp. DMC18

Annotation: Methanol dehydrogenase [cytochrome c] subunit 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 622 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 22 to 579 (558 residues), 624.6 bits, see alignment E=7.1e-192 PF01011: PQQ" amino acids 37 to 214 (178 residues), 67.9 bits, see alignment E=7.6e-23 amino acids 280 to 393 (114 residues), 56.1 bits, see alignment E=3e-19 amino acids 515 to 571 (57 residues), 42.5 bits, see alignment 3.8e-15 PF13360: PQQ_2" amino acids 84 to 220 (137 residues), 37.6 bits, see alignment E=2e-13

Best Hits

KEGG orthology group: None (inferred from 85% identity to meh:M301_1208)

Predicted SEED Role

"Methanol dehydrogenase large subunit protein (EC 1.1.99.8)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (622 amino acids)

>GFF2061 Methanol dehydrogenase [cytochrome c] subunit 1 (Methylophilus sp. DMC18)
MQQRIKLAISALLLGSISTSVWADAELDTLMKDDNQWAMQRKDYANQGYSRLSQINKGNV
KQLKMAWSFATGVNRGHEGAPLVIGNTMFIHTAFPNNVYALDLNDDQKILWSYFPKQSPD
VQSVMCCDNVNKGLGYGDGKILLQQNNGVLVALDAKTGAKVWEVTVNDPRTGAANTNAPH
VFKDKVITGCSGGEFGVRCHISAYNIKDGSLIWKAYSTGPDSEVMIGPDFNKENPHYSAL
SLYEDVNGGNKMGGSFKNLDKAQLKFPEANLGVRTWLKPQQQKDGWQNGGGPTWGWYSYD
PKLNLMYYGSGNPGTWNPDVRPGDNKWSMTIFARDLDTGLARWGYQMTPHDEWDYDGMNE
IVLWEANGKQLATHFDRNGFGYTFDRTNGTLLVAEKMHPFVNWATKIDLKTGIPVKDPKY
STHQDYNAKGVCPSALGVKDEQPSAFSPKTKTFYVPLNHVCMTYEPVESKYVAGQPWVGA
TLTMFAGPDGVMGGFMAWDGLKGKAQWYNKEKFSAWGGALVTASDLVFYGTLDRWVKALD
AKTGKELWKFQVGSGVVGNMITYGHKGKQYVGVLSGIGGWAAVAMNLGLTNDTDGLGAAG
GYKELTKYNAAPGGGAMNVFSL