Protein Info for GFF2060 in Xanthobacter sp. DMC5

Annotation: NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 transmembrane" amino acids 12 to 29 (18 residues), see Phobius details amino acids 36 to 57 (22 residues), see Phobius details amino acids 71 to 93 (23 residues), see Phobius details amino acids 106 to 125 (20 residues), see Phobius details amino acids 131 to 150 (20 residues), see Phobius details amino acids 162 to 184 (23 residues), see Phobius details amino acids 210 to 235 (26 residues), see Phobius details amino acids 247 to 266 (20 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 316 to 337 (22 residues), see Phobius details amino acids 377 to 403 (27 residues), see Phobius details amino acids 413 to 438 (26 residues), see Phobius details amino acids 451 to 469 (19 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 126 to 421 (296 residues), 172.1 bits, see alignment E=7.9e-55

Best Hits

Swiss-Prot: 61% identical to HYFF_METI4: Hydrogenase-4 component F homolog (hyfF) from Methylacidiphilum infernorum (isolate V4)

KEGG orthology group: K12141, hydrogenase-4 component F [EC: 1.-.-.-] (inferred from 86% identity to xau:Xaut_0169)

Predicted SEED Role

"Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>GFF2060 NAD(P)H-quinone oxidoreductase subunit 2, chloroplastic (Xanthobacter sp. DMC5)
MNLLSGLLVDPAPLLVAVPAASAVLLALVPSYRVGAFLNVAACGLTFALALLLLGAPRIQ
GPFLIVDDLNIVFVVLNTFVAFTTAWFSAGYIAHELETGRLTPLNLRFYHAMYQVLLGAM
TLALLSNNLGVMWVAIEVATLTTVLMVGLYRTEAAIEAAWKYFMLGSVGIALALFGTILV
YMVAQPVVGEGTDGMIWSVLMKHAREFDPALLNVAFVFLLLGYGTKVGLAPLHAWLPDAH
SEGPTPISAVLSGLLLNVALFAVLRFKSLLAAHPGTLAPGPLMMAMGLGSLLLAGFMLYR
RRDIKRLFAYSSIEHMGIIAFAFGLGGPLANFAGLLHMTMHSLTKSAIFFAVGHACQVKG
TQELAKIRGLTTSHPLLGWSLVAGVVAIAGLPPAGVFMSEFLVVTSAFAARPWLALILVL
GLLIAFGALMVKLSAIAFGEPSPGTDPVKASYVPMGVHLALVFAAGLYLPPELVAWFRHV
AAVLG