Protein Info for GFF2057 in Xanthobacter sp. DMC5

Annotation: Hydrogenase-4 component B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 673 transmembrane" amino acids 6 to 30 (25 residues), see Phobius details amino acids 38 to 60 (23 residues), see Phobius details amino acids 82 to 103 (22 residues), see Phobius details amino acids 115 to 133 (19 residues), see Phobius details amino acids 138 to 155 (18 residues), see Phobius details amino acids 167 to 187 (21 residues), see Phobius details amino acids 207 to 232 (26 residues), see Phobius details amino acids 244 to 261 (18 residues), see Phobius details amino acids 273 to 296 (24 residues), see Phobius details amino acids 303 to 325 (23 residues), see Phobius details amino acids 329 to 366 (38 residues), see Phobius details amino acids 379 to 401 (23 residues), see Phobius details amino acids 431 to 456 (26 residues), see Phobius details amino acids 476 to 496 (21 residues), see Phobius details amino acids 533 to 553 (21 residues), see Phobius details amino acids 654 to 672 (19 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 133 to 416 (284 residues), 172.4 bits, see alignment E=6.6e-55

Best Hits

KEGG orthology group: K05903, NADH dehydrogenase (quinone) [EC: 1.6.99.5] (inferred from 82% identity to xau:Xaut_0166)

Predicted SEED Role

"Hydrogenase-4 component B / Formate hydrogenlyase subunit 3" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.99.5

Use Curated BLAST to search for 1.6.99.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (673 amino acids)

>GFF2057 Hydrogenase-4 component B (Xanthobacter sp. DMC5)
MTLAPTFQLLVVLAAVAALLLLAVAGAALARGPYGRPVVYGGALAVTLLAAVSALTAIPA
PTGDLVLPLGIPWIGANLRLDALSAVFLFVVNLGGAAASLYGLGYGRHDHAPERVLPFFP
AFLAGMSLVPLAADAYTFLLAWEFMSLASWALVVSHDKEAGNVQAGLVYILMASFGTLAL
MLAFGLLAGSGGGFTFEAMRAAPPTGWAGAVVLALAMLGAGSKAGLVPLHVWLPLAHPAA
PSHVSALMSGVMTKVAVYGFIRIVFDLAGPPDWWWGIPVIVAGAIAGVVGVLQALLQSDT
KRVLAFSTIENIGLIFVGLGLALAFKASGFGGAAALAFTAALFHVFNHAAMKSLLFFGAG
AVLTATGSRVMDRLGGLIHRMPVTSVAMLIGAAAISALPPLNGFASEWLVFQAILLSPEL
PAWGLKLSVPAAGALLALAAALAAACFVRLYGIVFLGRARSEQAANAHEVDAFSRAAMLA
LAGICVLAGLFPGLVIDALQPAVTALVGVRMPAQSEVAWLSIVPIAESRSSYNGLLVFLF
IASSALLTAFIIHRLATGAVRRAPPWDCGFPNADPVTQYSAGSFAQPIRRVFARTLFAAR
ERVEMPPPGSLAPARIEITARDPAWAFLFLPLAGLVERIATRANRLQYLTIRQYLALVFG
ALVLLLLVLALWT