Protein Info for GFF2057 in Variovorax sp. SCN45

Annotation: 23S rRNA (adenine(2030)-N(6))-methyltransferase (EC 2.1.1.266)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF04378: RsmJ" amino acids 34 to 76 (43 residues), 32.2 bits, see alignment 3.5e-12 amino acids 101 to 310 (210 residues), 152.9 bits, see alignment E=5.2e-49

Best Hits

KEGG orthology group: K07115, (no description) (inferred from 90% identity to vap:Vapar_4679)

Predicted SEED Role

"Protein involved in catabolism of external DNA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>GFF2057 23S rRNA (adenine(2030)-N(6))-methyltransferase (EC 2.1.1.266) (Variovorax sp. SCN45)
MFSYRHAFHAGNHADVLKHTVLIATLDHLLEKDAALTVVDTHAGAGLYRLDGDYAGTSGE
AAEGILRLLSGKKEPAAPASAPAPAAKSAAKKAAPEAEAPLADAIARYLGVISDFNSKGG
ARVYPGSPFIIQHLLRDHDKLKLFELHPTDSRTLSANIAQLEAGRQIAVLLEDGFGSATK
FLPPPSRRALVLMDPSYEMKTDYGRVLDFAAEALKRFATGTYAIWYPIIPRPEAHDLPRR
LKTMATKAGKSWLHATLTVKSSKITKDAASGETQRPGLPASGMFLINPPYTLKPLLAEAL
PQLVERLGQDRNATFSLESGG