Protein Info for GFF2053 in Variovorax sp. SCN45

Annotation: Adenosylhomocysteinase (EC 3.3.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05221: AdoHcyase
  • amino acids 15 to 478 (464 residues), 470 bits, E=5.8e-145
• TIGR00936: adenosylhomocysteinase
  • amino acids 17 to 471 (455 residues), 592.1 bits, E=2.8e-182
• PF00670: AdoHcyase_NAD
  • amino acids 239 to 398 (160 residues), 270.8 bits, E=9.1e-85
• PF02826: 2-Hacid_dh_C
  • amino acids 258 to 348 (91 residues), 33.6 bits, E=5e-12
• PF07991: IlvN
  • amino acids 259 to 341 (83 residues), 21.8 bits, E=2.5e-08

Best Hits

Swiss-Prot: 96% identical to SAHH_VARPS: Adenosylhomocysteinase (ahcY) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01251, adenosylhomocysteinase [EC: 3.3.1.1] (inferred from 98% identity to vpe:Varpa_5312)

Predicted SEED Role

"Adenosylhomocysteinase (EC 3.3.1.1)" in subsystem Methionine Biosynthesis or Methionine Degradation (EC 3.3.1.1)

MetaCyc Pathways

• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine degradation I (to L-homocysteine) (2/3 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (3/5 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Selenoamino acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.1.1

Protein Sequence (479 amino acids)

>GFF2053 Adenosylhomocysteinase (EC 3.3.1.1) (Variovorax sp. SCN45)
MSAVLKPTPVSTADQAIADLSLAAWGRKEIRIAETEMPGLMAIREEFSKGQALKGARITG
SLHMTIQTAVLIETLQALGAEVRWASCNIFSTQDHAAAAIAEKGTPVFAIKGESLKDYWD
YTHAIFDFGPKGSKGEGPNMILDDGGDATLLMHLGQRAEKDLGVLANPTSEEERILFAAI
KAKLAVDDTWYTRKSAEIIGVTEETTTGVHRLNEMSAKGTLLFRAINVNDSVTKSKFDNL
YGCRESLVDGIKRATDVMIAGKVACVAGYGDVGKGSAQALRALSAQVWVTEIDPINALQA
AMEGYKVVTMEYAADKADIFVTTTGNKDVITHDTMVKMKDQAIVCNIGHFDNEIDVASIE
KYEWEEIKPQVDHITFPDGKKIILLAKGRLVNLGCGTGHPSFVMSSSFANQTIAQIELFT
KPDAYQAGKVYVLPKHLDEKVARLHLKKVGAMLTELTDAQAAYIGVSKNGPYKPDTYRY