Protein Info for GFF2052 in Sphingobium sp. HT1-2

Annotation: Phosphoserine phosphatase (EC 3.1.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 TIGR00338: phosphoserine phosphatase SerB" amino acids 73 to 279 (207 residues), 208.2 bits, see alignment E=1.2e-65 TIGR01488: HAD phosphoserine phosphatase-like hydrolase, family IB" amino acids 79 to 248 (170 residues), 96 bits, see alignment E=2.6e-31 PF00702: Hydrolase" amino acids 80 to 251 (172 residues), 69.2 bits, see alignment E=1e-22 PF12710: HAD" amino acids 81 to 248 (168 residues), 73.4 bits, see alignment E=5.2e-24 PF08282: Hydrolase_3" amino acids 226 to 270 (45 residues), 27.9 bits, see alignment 2.9e-10

Best Hits

KEGG orthology group: K01079, phosphoserine phosphatase [EC: 3.1.3.3] (inferred from 89% identity to sjp:SJA_C1-14660)

Predicted SEED Role

"Phosphoserine phosphatase (EC 3.1.3.3)" in subsystem Glycine and Serine Utilization or Serine Biosynthesis (EC 3.1.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.3

Use Curated BLAST to search for 3.1.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>GFF2052 Phosphoserine phosphatase (EC 3.1.3.3) (Sphingobium sp. HT1-2)
MFVATLVAVETLKQADIAEAAGRLASAGCAPVDSVWLDEGKAADIFFGSDPVTARAALTD
IGYRVDVIVQMAAGREKTLLIADMDSTMITVECIDELADYAGIKPQIAEITERAMRGELD
FEGALHGRVALLKGLPDSAIDQCREERVVIMDGARELVRTMKARGARTLLVSGGFTRFTG
PVAAEIGFDVHVANVLEIADGALLGTVTVPIVDAARKRTELEAAIDGGIDRALTLAVGDG
ANDIPMIEGAGLGVAYHAKPKTRAAAAAEIVHGDLSVLLYAQGIASADWVRD