Protein Info for GFF2052 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 778 transmembrane" amino acids 112 to 131 (20 residues), see Phobius details amino acids 142 to 161 (20 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 205 to 223 (19 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details amino acids 393 to 416 (24 residues), see Phobius details amino acids 728 to 746 (19 residues), see Phobius details amino acids 752 to 770 (19 residues), see Phobius details PF00403: HMA" amino acids 30 to 90 (61 residues), 57.2 bits, see alignment 2.8e-19 TIGR01525: heavy metal translocating P-type ATPase" amino acids 175 to 770 (596 residues), 565.8 bits, see alignment E=2.5e-173 TIGR01494: HAD ATPase, P-type, family IC" amino acids 213 to 752 (540 residues), 258.6 bits, see alignment E=1.8e-80 PF00122: E1-E2_ATPase" amino acids 241 to 345 (105 residues), 88.4 bits, see alignment E=3.6e-29 PF00702: Hydrolase" amino acids 440 to 678 (239 residues), 108.8 bits, see alignment E=7.3e-35

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 68% identity to adn:Alide_0037)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (778 amino acids)

>GFF2052 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MLTFMNTVTAPADAGASFSTYPLTQIDLGIGGMTCASCVTRVEKALRKQPGVNDATVNLA
TESARVTLSGVDEAESLARIKRAVRDAGYEPRTLEAMDEVDTERVMGVPRDALPVLIGVL
LSAPLVVPMVGDLFGQHWMLPAWIQFLLATPVQFVLGARFYRAGWHALKARTGNMELLVA
IGTTAGWALSTWLWWRAEAGEMPHLYYEASAVVITLVLLGKWLEARAKRQTTAAIRALHA
LRPDRAHLLPDGIRRTETTDVAVDELLPGDVIRVLPGERFAADGTVVQGHTQADESMLTG
ESLPVPKDVGDAVTGGSLNGEGAVDVRVRAVGAQSVLAQIIGLVQDAQAGKAPVQRMVDQ
VAAVFVPVVLVIALVTLLGWLWTGAPMEEALLHAVAVLVIACPCALGLATPAAIMAGTGV
AAQHGILIKDAQALEIAHKVDTVAFDKTGTLTEGHPRLTVIEAAPGVDEIAVLQVAAAMQ
AQSAHPLARAVLEAAASRSIEVAADAAQDVQAVSGRGSQGRVNGALLALGSLRWMGELKV
VLGPLEERARVLQADGATVSVLASQTPAHGTAAATWSPLALLAFGDEPKVGAAEAIAGLR
AQGMRLFMVSGDNRGAALAMAARLGLRADHDEVIAEVLPGDKATVVRKLRAAVVGDRLHT
VAMVGDGVNDAPALAAADVGMAMSHSQGASADVAMHAAGITLMRGDPLLVAAALDISRRT
VRKIRQNLFWAFAYNVAGIPLAALGFLSPVVAGAAMALSSVSVVSNALLLKRWKPPQR