Protein Info for GFF2050 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Pyridoxal kinase (EC 2.7.1.35)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 21 to 284 (264 residues), 168.6 bits, E=9.7e-54
• PF08543: Phos_pyr_kin
  • amino acids 93 to 270 (178 residues), 82.1 bits, E=4.2e-27
• PF00294: PfkB
  • amino acids 151 to 261 (111 residues), 43.2 bits, E=3.4e-15

Best Hits

Swiss-Prot: 100% identical to PDXK_SALTY: Pyridoxine/pyridoxal/pyridoxamine kinase (pdxK) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 99% identity to spt:SPA0430)

MetaCyc: 68% identical to pyridoxine/pyridoxal/pyridoxamine kinase (Escherichia coli K-12 substr. MG1655)
Hydroxymethylpyrimidine kinase. [EC: 2.7.1.49]; Pyridoxal kinase. [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]; 2.7.1.35 [EC: 2.7.1.49, 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• thiamine diphosphate salvage II (5/5 steps found)
• pyridoxal 5'-phosphate salvage I (5/6 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (6/9 steps found)
• thiamine diphosphate salvage IV (yeast) (4/7 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism
• Vitamin B6 metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.35, 2.7.1.49

Use Curated BLAST to search for 2.7.1.35 or 2.7.1.49

Protein Sequence (288 amino acids)

>GFF2050 Pyridoxal kinase (EC 2.7.1.35) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MGQESDIQSVLFDDNHRALQTDIVAVQSQVVYGSVGNSIAVPAIKAQGLRVTAVPTVLFS
NTPHYKTFYGGIIPAEWFAGYLTALNERDALRELKAITTGYMGSADQIVLLSKWLMAIRA
SHPEVCILVDPVIGDTDSGMYVQAEIPQAYRTHLLPQAQGLTPNVFELEMLSGKPCRTLE
EAVAAAQSLLSDTLKWVVITSAPGESLETITVAVVTAQVVEVFAHPRVATELKGTGDLFC
AELVSGIVQGKKLTTAAKDAAQRVLEVMTWTQQCGCDELILPPAGEAR