Protein Info for GFF2044 in Sphingobium sp. HT1-2

Annotation: Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 81% identical to GATA_SPHWW: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 94% identity to sjp:SJA_C1-22090)

Predicted SEED Role

"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)

MetaCyc Pathways

L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF2044 Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7) (Sphingobium sp. HT1-2)
MTYLTDLTVAEIRDGFRAGDFSAREVAEGFNANVAAAKALNAFIVETPEKALAAADAADK
AKAAGETLGQLAGVPIGMKDLFCTEGTQTTAASHMLEGFVPTYESTVSGKLWAAGAGMLG
KLNLDQFAMGSSNETSYYGNVISPWRRGGGDNAALAPGGSSGGSSSAISARLCPAATGTD
TGGSIRQPAAFTGISGIKPTYGRCSRFGIVAFASSLDQAGPMARTVRDNAILLEVMSGFD
PKDSTSLDLAVPQWEANLSSDLKGKKVGIPKEYRPDGLNAEISAMWDRGIEWLKDAGAEV
VDVSLPHTKYALPTYYIIAPAEASSNLARYDGVRYGQRDLPDGAGLQDMYAATRAAGFGP
EVKRRIMIGTYVLSAGFYDAYYTQAQKVRALIARDFELAFEKCDLLLTPTAPSASFALGE
KQADPLAMYLNDVFTVPASLAGLPAMAIPGGLDSAGLPIGLQVIGKALDEQTVLNASLAI
EERVGFSARPDKWW