Protein Info for GFF2037 in Sphingobium sp. HT1-2

Annotation: Glycogen phosphorylase (EC 2.4.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 821 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 21 to 819 (799 residues), 1139 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 108 to 820 (713 residues), 1039.7 bits, see alignment E=0

Best Hits

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 90% identity to sch:Sphch_0584)

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (821 amino acids)

>GFF2037 Glycogen phosphorylase (EC 2.4.1.1) (Sphingobium sp. HT1-2)
MNLKGETQLPKPAPRQVDPQILAHEIVERLTYRIGKDAKAAKPHDWLHAVILSIRDRVID
AWIESTHKTYEEQGRRVYYLSLEFLIGRLMRDAASNMEMLDDLQAALDSLGVDLDLIAAL
EPDAALGNGGLGRLAACFMESMATVDIPAYGYGIRYVNGMFRQEISDGWQVELPENWLAH
GNPWEFERREASYEVGFGGRVDPAECENCGPYQMSWRPAERVIATPYDTPIAGWRGKRVN
TLRLWEAQPIDPILLDKFNAGDHLGALSESNRAEALTRVLYPADSSPAGQELRLRQEYFF
SSASLQDIVRRHIQYFGDVRTLPDKAAIQLNDTHPAVSVAELMRILVDEHGLDFDEGWDI
VRRTFGYTNHTLLPEALESWPVPLFERLLPRHMQIVYAINSRLLGEARKSGQFDDHAIGT
ISLIDEGGERRVRMGNLAFAGSHSVNGVSALHTDLMKVTVFADLHKLYPARINNKTNGVT
FRRWLMQCNHGLFELIREAIGDRFMDDAEALRELDNFADDTAFQERFLAVKRFNKVALAD
LLRKRINARIDADALFDIQIKRIHEYKRQLLNIIEAVALYDQIRSHPEKDWVPRVKLFGG
KAAPSYHNAKLIIKLAGDVARAINHDPSVQGLLKVQFVPNYNVSMAEMMIPAADLSEQIS
TAGMEASGTGNMKFAVNGALTIGTLDGANVEMRDHVGEDNIVIFGLTAAEVNQRRAEGYN
PRAVIEQSRELGQALNAIASGVFSPDDPNRYKDLIQGIYDHDWFMVAADFDSYAAAQRRV
DGIWQDQALWAKKAIHNVARMGWFSSDRTIREYAADIWNMG