Protein Info for GFF2035 in Methylophilus sp. DMC18

Annotation: Biotin carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit" amino acids 1 to 445 (445 residues), 755.9 bits, see alignment E=7.2e-232 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 158.5 bits, see alignment E=2.2e-50 PF02655: ATP-grasp_3" amino acids 114 to 294 (181 residues), 26.6 bits, see alignment E=1.8e-09 PF02786: CPSase_L_D2" amino acids 115 to 322 (208 residues), 274.3 bits, see alignment E=1.9e-85 PF02222: ATP-grasp" amino acids 140 to 292 (153 residues), 37.2 bits, see alignment E=7.2e-13 PF07478: Dala_Dala_lig_C" amino acids 145 to 291 (147 residues), 30.8 bits, see alignment E=6.3e-11 PF02785: Biotin_carb_C" amino acids 336 to 442 (107 residues), 130.7 bits, see alignment E=7.2e-42

Best Hits

Swiss-Prot: 73% identical to ACCC_ALLVD: Biotin carboxylase (accC) from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 89% identity to mmb:Mmol_0155)

MetaCyc: 71% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>GFF2035 Biotin carboxylase (Methylophilus sp. DMC18)
MFEKILIANRGEIALRVQRACRELGIKTVAVHSEADRDAKYVKLADESVCIGPAPSAKSY
LNIPAVISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVFIGPRAETIRLMGDKVSAK
DSMKAAGVPCVPGSDGALNDDPAEIIKTAKRVGYPVIIKAAGGGGGRGMRVVHTEAALLN
AVNMTKAEAQAAFGNPMVYMEKFLENPRHIEIQILADQHGNAVFLGDRDCSMQRRHQKII
EEAPSPLLPERLRDKIGERCAEACRKIGYRGAGTFEFLYENGEFYFIEMNTRLQVEHTVT
EMITGIDLVQQQIFIAAGEKLPFRQRDIVLNGHAIECRINAEDPYTFVPSPGFINRFHMP
GGPGVRMDTHVYGGYTVPPHYDSMIGKLITHGATREQAIARMRVALSEMYVEGIKTNTAL
HSDLMADLAFQQGGTSIHYLEQKLGISSK