Protein Info for PS417_10350 in Pseudomonas simiae WCS417

Annotation: multidrug MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF06441: EHN" amino acids 36 to 141 (106 residues), 133.6 bits, see alignment E=3.1e-43 PF00561: Abhydrolase_1" amino acids 127 to 235 (109 residues), 30.3 bits, see alignment E=3.3e-11

Best Hits

Swiss-Prot: 53% identical to HYEP_STIAD: Putative epoxide hydrolase (STAUR_4299) from Stigmatella aurantiaca (strain DW4/3-1)

KEGG orthology group: None (inferred from 86% identity to pba:PSEBR_a2849)

Predicted SEED Role

"Epoxide hydrolase (EC 3.3.2.9)" (EC 3.3.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.3.2.9

Use Curated BLAST to search for 3.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UN83 at UniProt or InterPro

Protein Sequence (430 amino acids)

>PS417_10350 multidrug MFS transporter (Pseudomonas simiae WCS417)
MLAASSLYLRLRCRASLLGLALLAGSQAAWAASDAIRPYRIHVDEAQLADLHKRIAATRW
PDKETVNDTSQGVQLAQVQALVKYWGNGYDWRKAEARLNALPEFITTIDGVDIQFIHVRS
RNPNALPLILTHGWPGSQFEFLKTIGPLTDPVAYGGRIEDSFDVVIPSIPGHGFSGKPTE
LGWGPDRVAKAWDVLMKRLGYSHYVSQGGDHGSVISDALGRLSPPGLLGIHLNMPATVPP
ELVKPINSGEPAPATLTEPERTAYNALSTFFGRNAAYGAMMVTRPQTIGYLLADSAAGTA
AWMYEKFAAWTDSDGKPERVLSRDEMLDDISLYWLTNSGAASSRFYWENNNNNFSAAAQK
TDTIKVPVGITVFPHEIYQAPKSWAQRAYPSLSYYSQVSKGGHFAAWEQPQLFSEELREA
FRPLRPAAKP