Protein Info for GFF2025 in Sphingobium sp. HT1-2

Annotation: beta-galactosidase (EC 3.2.1.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1031 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF22666: Glyco_hydro_2_N2" amino acids 93 to 186 (94 residues), 41.8 bits, see alignment E=3.1e-14 PF02837: Glyco_hydro_2_N" amino acids 96 to 203 (108 residues), 50.2 bits, see alignment E=7.6e-17 PF00703: Glyco_hydro_2" amino acids 235 to 330 (96 residues), 49.4 bits, see alignment E=1.8e-16 PF16355: DUF4982" amino acids 630 to 687 (58 residues), 53.4 bits, see alignment 4.9e-18

Best Hits

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1031 amino acids)

>GFF2025 beta-galactosidase (EC 3.2.1.23) (Sphingobium sp. HT1-2)
MKRKSAVGARVRQLRGGLALLALTPLLPLAPAAAHDASTVTASPRQTYNLNPGWLMTTGD
QAGAQQPGFDDKGWQSVTLPNAFNETQAFARDIKALSTGIIWYRKHISLPADATRGRAFL
EFEGVRQAAEIWVNGRSVALSEHGVMAFGADITAALKPGDNVIAVRVDNDWKYKERATGS
GFQWNNDNFNVNYGGITKNVRLHLTGPVYQTLPLYSSLGTTGQYVWADGFDIAGRRANIH
AESQVRNDSAAAHALSLRVEIRDPAGRRIARFDSAATSIAPGQSAALAAAAPVADLHYWS
WGYGYLYTVTTSLMDKGKVIDSVDTKTGFRQTRFGDGKVDLNGRTIMVHGYAQRTSNEWP
AVGTSIPPWITDFSNALMVESGGNLVRWMHVTPSKQDIESADRVGLMQAMPAGDAESDVT
GRRWDQRKEVMRDAIIYNRNNPSILFYESGNENISEAHMAEMKAIRDQYDPHGGRAIGSR
EMLDSKVAEYGGEMMYINKSAKHPMWSMEYSRDEAARAYQDNFTPPFHKDAPAYNRNQDS
HAIEDVKRWNDYYVMRPGTGTRVSSGGVNIIWSDSNTHFRGDNNYRRSGEVDAVRIPKQG
FFAHQVMWDDWVDSVTPATHIIGHWNYAPGTVKDMSVVSNGDSVELFLNGRSLGKGQRSD
SFLFSWKAIAFAPGMLRAVATHADGKTSEFSLSTTGPAVALKLTPHVSPRGFVMDGADIA
LVDVEAVDAQGRRMPVDASKVHFDLKGPALWRGGIAQGDSQGKVRAGQTEGEAVVTKAHG
ADGTTSYAGTAREEDNYILSPDLPLEGGVNRVLIRAGTKAGTVSLTATAPGLKAASLSIA
TKLPAATKGGLSTDFADAFQPGSLVRGPTPATPSFTPHRTTFVAQDIRAGSGQADAGATV
DDNELTRWASDGKPDNGWIEYRFATPVHLSEIDLKLVGWRSRAYPLELTIDGKSVWRGET
ERQLGYTTLRFPPTTGQTLRIRQTGPVQDKDAFGKVVELASARQAGDTGADAVPPGWHLG
IVEAEFHGPVD