Protein Info for GFF2014 in Sphingobium sp. HT1-2

Annotation: Glycerol kinase (EC 2.7.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF00370: FGGY_N" amino acids 7 to 253 (247 residues), 244.2 bits, see alignment E=1.6e-76 TIGR01311: glycerol kinase" amino acids 7 to 495 (489 residues), 695.1 bits, see alignment E=2e-213 PF02782: FGGY_C" amino acids 263 to 451 (189 residues), 140.8 bits, see alignment E=4.9e-45

Best Hits

Swiss-Prot: 65% identical to GLPK_GLUOX: Glycerol kinase (glpK) from Gluconobacter oxydans (strain 621H)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 69% identity to apt:APA01_12552)

MetaCyc: 54% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (496 amino acids)

>GFF2014 Glycerol kinase (EC 2.7.1.30) (Sphingobium sp. HT1-2)
MPQPVHILAIDQGTTSTRAIVFDRHARPVASAQCEFEQHYPDLGWVEHDPEDIWRDTLRL
AKEAIEKSGVGTGGIAGIGITNQRETVVVWERGTGKVIHRAIVWQDRRTARLCAELKGEE
GFEAEVRSRTGLLVDPYFSGTKLAWILDNVGGARERAERGELAFGTIDSFLIWRLTGGKV
HATDVTNAGRTMLYNIREQRWDEEMCRLLRVPMQVLPEVHDNAHHFGTTDAALLGAAIPI
AGVAGDQQAALFGQACFGVGMAKSTYGTGCFMLLNTGTEALESNNRLLTTPAYRLNGQMT
YALEGSIFVAGAAVKWLRDGIGVITHARETNDMATRVPSSHGVYMVPAFVGLGAPHWDPD
ARGAIFGLTLDATQAHLARAALEAVAYQTMDLAEAMVSDGGAAPAKLRVDGGMAANDWLC
QFLADMLETKVERPVHLETTALGAAFHAGLATGLWADLNALAETWTQGQIFEPKMANFER
KTLVAGWQDAVRRTLR