Protein Info for GFF2013 in Xanthobacter sp. DMC5

Annotation: Vitamin B12 transporter BtuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 852 transmembrane" amino acids 42 to 65 (24 residues), see Phobius details PF07715: Plug" amino acids 97 to 208 (112 residues), 40.2 bits, see alignment E=4.3e-14 PF00593: TonB_dep_Rec_b-barrel" amino acids 371 to 811 (441 residues), 138.6 bits, see alignment E=5.9e-44

Best Hits

KEGG orthology group: None (inferred from 79% identity to xau:Xaut_1786)

Predicted SEED Role

"Outer membrane receptor proteins, mostly Fe transport" in subsystem Hemin transport system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (852 amino acids)

>GFF2013 Vitamin B12 transporter BtuB (Xanthobacter sp. DMC5)
MHGERESGASRSAHSQRVASRREAPGKSTAGKTAAASASRRAMAVALGLSAAAVPVALLA
AFPAVAQEQSDSSGTLQLGTVEVVATTPLSGTGIDADKVPGSVTIIGADQFDRLNSFAVT
DGLFQYTPSANTSDVQGNAFFQDFRYRGFAASPLQGTPQGIAVYQNGIRLNEAFGDTVNW
DLIPEVAISRAEVFSNNPAFGLNALGGAVSIQMKNGFNYHGFEGSIQGGSFGQIGGNIQY
GGEKDGFGVYIAADAVHSDGWRLQSPSDLARFYGDLGWKNDKTEVHLVASAATTNLGVVG
PTPVEMLNQNWNSIYTWPQTTNNDVGMLALNGRYDLTDTWQLQGNLYARKFSQSHVDGND
ADLEACSNKWNPGVGNTKMCLEEDQFTGPPGGGNPATNPTYAAFRSQMVLYDQNGNTIPF
TALGPNQLYGVINRTWTDTTTYGGSVQATSTDKLLGHDNYFVMGASVDSSDINFRSNTEL
GITNTSTLQVVGTGTYLKNIPNYSLPDQYQVFYLPVDLNASTRYYGLYATNAFDMTDRAT
LTMGARLNIAQISMQDGTGTSPQLNGDYTFARINPLIGLTYKLTPEATLYGGYSESNRAP
TPLELNCADPNRPCLLENSLVADPPLNQVVSHTYEVGLRGNGMLFGGKVNWKLGVYRVDS
DDDIVSLASQQTGYGYYTNVPATRRQGFEAEMQYVNGPWKFYANYAYLNATYQFTGYLAS
PNNPYANDGQVLVTPGDTIPGIPTQTLKLGVDYMVTPKWKLGGDVIAVSSQYYVGDDANQ
APMLPGYWVANIHTSYQVTDNIQVFGYINNLFNAKYATYGTFFDTEYLGLNNPQMQSPGQ
PFSAYAGLKATF