Protein Info for GFF2004 in Xanthobacter sp. DMC5

Annotation: Caffeine dehydrogenase subunit gamma

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 15 to 62 (48 residues), 30.8 bits, E=2.3e-11
• PF01799: Fer2_2
  • amino acids 82 to 155 (74 residues), 93.6 bits, E=6.5e-31

Best Hits

Swiss-Prot: 51% identical to CDHC_PSEU3: Caffeine dehydrogenase subunit gamma (cdhC) from Pseudomonas sp. (strain CBB1)

KEGG orthology group: K03518, carbon-monoxide dehydrogenase small subunit [EC: 1.2.99.2] (inferred from 79% identity to rpc:RPC_0850)

MetaCyc: 51% identical to 1-testosterone hydratase/dehydrogenase gamma subunit (Steroidobacter denitrificans)
RXN-21494 [EC: 1.17.99.11]; 1.17.99.11 [EC: 1.17.99.11]; 1.17.99.11 [EC: 1.17.99.11]

Predicted SEED Role

"Carbon monoxide dehydrogenase small chain (EC 1.2.99.2)" in subsystem CO Dehydrogenase (EC 1.2.99.2)

MetaCyc Pathways

• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Trinitrotoluene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.17.99.11 or 1.2.99.2

Protein Sequence (172 amino acids)

>GFF2004 Caffeine dehydrogenase subunit gamma (Xanthobacter sp. DMC5)
MLRLSAAARHTVRFTLNGRPVVGQAAPRTLLSDFLRHEIGATGTHVGCEHGVCGACTVDV
DGMLTRACMTLAVQVEGATIRTVEGLAPAPDRLSVLQAAFRRHHALQCGFCTAGILMSLD
VFLKAEPDPSEEAVRDLLSGHLCRCTGYTPIVRAALEAAAELRDAQGESAHA