Protein Info for GFF2004 in Sphingobium sp. HT1-2

Annotation: tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 68% identity to sch:Sphch_1599)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (315 amino acids)

>GFF2004 tRNA(Ile)-lysidine synthetase (EC 6.3.4.19) (Sphingobium sp. HT1-2)
VPELAPSHRPDALRLAVRALVDGEETARFGIAVSGGPDSMALLDLAVQAWPGRVAAATLD
HGLRAESAAEAAMVARWCAERRVPHATLAPETAVSGNIQDWARRQRYAQLEAWRAGAGLD
WILTAHHADDQLETLLMRLNRGSGVGGLAGVRARAGRVLRPLLTVRKAQLQAHADQAGLP
YVLDPSNADPRFDRAALRKRLDGVDWIDAEAAARSAAALAEAEAALQWTVAGLAATHIHP
STDAWVLDRTDLPRELLRRLLIEMLCSADPEIVLPRGEALDRAIAAARAGRQASLGDWLL
RGGAVWTLSPAPPRR