Protein Info for GFF2002 in Sphingobium sp. HT1-2

Annotation: Cell division-associated, ATP-dependent zinc metalloprotease FtsH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 109 to 131 (23 residues), see Phobius details PF06480: FtsH_ext" amino acids 18 to 102 (85 residues), 57.2 bits, see alignment E=5.3e-19 TIGR01241: ATP-dependent metallopeptidase HflB" amino acids 112 to 605 (494 residues), 779.6 bits, see alignment E=6.4e-239 PF00004: AAA" amino acids 201 to 332 (132 residues), 157.7 bits, see alignment E=6.3e-50 PF07728: AAA_5" amino acids 201 to 321 (121 residues), 24.2 bits, see alignment E=8.8e-09 PF17862: AAA_lid_3" amino acids 355 to 399 (45 residues), 52.6 bits, see alignment 7.7e-18 PF01434: Peptidase_M41" amino acids 413 to 604 (192 residues), 240.3 bits, see alignment E=4.7e-75

Best Hits

Swiss-Prot: 66% identical to FTSH_ZYMMN: ATP-dependent zinc metalloprotease FtsH (ftsH) from Zymomonas mobilis subsp. mobilis (strain NCIMB 11163 / B70)

KEGG orthology group: K03798, cell division protease FtsH [EC: 3.4.24.-] (inferred from 96% identity to sjp:SJA_C1-24660)

MetaCyc: 65% identical to ATP-dependent zinc metalloprotease FtsH (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"Cell division protein FtsH (EC 3.4.24.-)" in subsystem Bacterial Cell Division (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (648 amino acids)

>GFF2002 Cell division-associated, ATP-dependent zinc metalloprotease FtsH (Sphingobium sp. HT1-2)
MNDEKDPQGNPWIKSAMIWAGVIIALLLFVSLFERPTTQAGSAIAYSEFRAKVQEGQVKD
VAIAPDRITGTLSSGQGFSTVPVNDPKLTDLLDDYNVKYSGQAEQQPSFWQIMLYQSLPF
LLILGIAFFVLRQMQKGGGAGGAMGFGKSKAKLLTEKHGKVTFDDVAGIDEAREELQEIV
EFLKDPSKFARLGGKIPKGALLVGSPGTGKTLLARAIAGEAGVPFFTISGSDFVEMFVGV
GASRVRDMFEQAKKNAPCIVFIDEIDAVGRHRGAGLGNGNDEREQTLNQLLVEMDGFEAN
EGIIIVAATNRPDVLDPALLRPGRFDRQVVVPRPDIEGREKILAVHMKKVPLAPDVNPRT
IARGTPGFSGADLANLVNEAALMAARRGKRLVAMDEFEAAKDKVMMGSERRSMVMTDDEK
KMTAYHEAGHAIVAVHEPASDPIHKATIIPRGRALGMVMRLPERDSYSYHRDKMHANMAV
AMGGRVAEEIIFGYDKVSSGASGDIQYATKLARDMVTQWGMSDKLGPLQYEEQQGETFLG
YSQSQRVHMSDETAKLIDKEIRGLVEQGYARAQELLKGHEDQLHLLANAMLEYETLSGEE
IKALLEKGEITRDDGTTIKPSVIPAAGSSIPKTRKRKGPFGDPTPAGA