Protein Info for GFF2000 in Variovorax sp. SCN45

Annotation: Phosphomethylpyrimidine synthase ThiC (EC 4.1.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 17 to 85 (69 residues), 78 bits, E=4e-26
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 132 to 587 (456 residues), 704.6 bits, E=2e-216
• PF01964: ThiC_Rad_SAM
  • amino acids 133 to 585 (453 residues), 619.2 bits, E=3.4e-190

Best Hits

Swiss-Prot: 95% identical to THIC_VARPS: Phosphomethylpyrimidine synthase (thiC) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 96% identity to vpe:Varpa_5365)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (627 amino acids)

>GFF2000 Phosphomethylpyrimidine synthase ThiC (EC 4.1.99.17) (Variovorax sp. SCN45)
MNAPDKFTSLLSLTREPFPASHKCLIPGSRPDLNVPVRDVRLTNGETVSLYDTSGPYTDP
KVEIDVRRGLPDVRGAWVVERGDTESYEGRTHHALDDGAKNEDRDAQRLAELRAGASALQ
RQPRRAKSGANVTQMHYARRGIVTPEMEYVALRENGKREWMAEYLANEERAKRVMGNPMG
ASIPRIITPEFVRDEVARGRAIIPANINHPEVEPMAIGRNFKVKINANIGNSAVTSSIEE
EVEKLVWAIRWGADNVMDLSTGKNIHTTRDWIVRNSPVPIGTVPIYQALEKVGGVAEDLT
WEIYRDTLIEQAEQGVDYFTIHAGLRLPFIHLTADRMTGIVSRGGSIMAKWCIAHHKESF
LYTHFEDICDIMKAYDVSFSLGDGLRPGSGADANDEAQFAELRTLGELTQIAWKHDVQTM
IEGPGHVPMHMIQSNMDEQLKHCHEAPFYTLGPLTIDIAPGYDHIASAIGAAMIGWAGTA
MLCYVTPKEHLGLPDRDDVKQGIIAYKIAAHAADVAKGHPGARSRDDALSKARFEFRWQD
QFNLGLDPDTAREFHDETLPKDSSKVAHFCSMCGPKFCSMKITQEVREYAAKKGVAEAEA
MTEGMEEKSKEFMAGGGEIYIPIKAVS