Protein Info for GFF1996 in Variovorax sp. SCN45
Annotation: Indoleacetamide hydrolase (EC 3.5.1.-)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to MANHY_PSEPU: Mandelamide hydrolase (mdlY) from Pseudomonas putida
KEGG orthology group: K01463, [EC: 3.5.1.-] (inferred from 89% identity to vpe:Varpa_5369)Predicted SEED Role
"Indoleacetamide hydrolase (EC 3.5.1.-)" in subsystem Aromatic amino acid degradation (EC 3.5.1.-)
MetaCyc Pathways
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (1/2 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Caprolactam degradation
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Lysine biosynthesis
- Nicotinate and nicotinamide metabolism
- Nucleotide sugars metabolism
- Sphingolipid metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.-
Use Curated BLAST to search for 3.5.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (506 amino acids)
>GFF1996 Indoleacetamide hydrolase (EC 3.5.1.-) (Variovorax sp. SCN45) MSISRSVPAAALALLCLGGLGLPAQAQQPAAAPDIATLTASDAVKQLCAGTITSEQLVAA YIAQAKARANLNAFIALDEAGALKQARAADALRKRGGACKPLAGLPVAIKDNIQVQGLPA TAGTPALKGFVPGADAPVVARLRAAGAVVLGKTNMHELAFGVTGYNPAFQSGPEVGVRNA YDASRIAGGSSSGNGAALGARMVPAALGTDTGGSVRIPCAFNGCASLRPSMGRYPQQGIA PISHTRDTAGPMAQSMADVALLDGVIAGGAPIRPADLKRVRLGVAQAFYANLDADTRAAT DAALARLRGAGVTLVDVEMPKLMELNGAIGFPLALYEAHDDMAAYLAKYRTGIDIAQLAA GIASPDVKGTFDAFVIPRKLPAPAGTVDAKPVYDNAMRVARPALQKLYRDTFAKNRLDAL VFPTVPRVALAAAPEASSPENFGALIQNTDPGSNAGIPGVQLPSGLGATTGLPVGLELDG PAGSDRKLLAVGLAVERVLGRLPAPK