Protein Info for GFF1995 in Sphingobium sp. HT1-2

Annotation: sigma-54 dependent DNA-binding response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 PF00072: Response_reg" amino acids 10 to 121 (112 residues), 70.6 bits, see alignment E=4.3e-23 PF00158: Sigma54_activat" amino acids 150 to 315 (166 residues), 228 bits, see alignment E=2e-71 PF14532: Sigma54_activ_2" amino acids 164 to 320 (157 residues), 63.6 bits, see alignment E=8.2e-21 PF07728: AAA_5" amino acids 172 to 291 (120 residues), 29.8 bits, see alignment E=1.9e-10 PF02954: HTH_8" amino acids 432 to 471 (40 residues), 46 bits, see alignment 1.2e-15

Best Hits

KEGG orthology group: None (inferred from 87% identity to sjp:SJA_C1-25650)

Predicted SEED Role

"Sigma-54 dependent DNA-binding response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (478 amino acids)

>GFF1995 sigma-54 dependent DNA-binding response regulator (Sphingobium sp. HT1-2)
MVREGVPLLMLIDDEPAQRRLVSALAARAGWRTIFASDAETALATLGTQDGIQLDAVLID
QWSPDFDPADLIGDIHAQRPALPILVLTAHNSVAVAVQAMRAGATDYLSKPIAPERLLAA
LNATLEGNVRGELRPLTEKISAPLAFEDVVGAAPQFRTALAIAAKAARARVPVLIEGESG
VGKDVVARAIHAASPRHKQAMVTVNCGAIPANLVESELFGHERGAFTGAFDRHVGKFLSA
DMGTIFLDEVSEMPLDAQVKLLRVLQDGEVQPIGARRPVHVDVRVIAATNKKLSDEIEAG
RFREDLYYRLNVVQLTVPPLRERMGDIPALCRHLLARIATQPGLRGLGLTDDALALLMQH
DWPGNVRQLQNALFRAAVLCEGDALTPHDFPQVAAHILSRGASGPRQRAAATPPREAAGI
TLFEGDGHVRQLAEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLAELGIDNAA