Protein Info for GFF1990 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Acetoacetyl-CoA synthetase (EC 6.2.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 TIGR01217: acetoacetate-CoA ligase" amino acids 8 to 689 (682 residues), 650.7 bits, see alignment E=1e-199 PF16177: ACAS_N" amino acids 27 to 81 (55 residues), 44.2 bits, see alignment 2.1e-15 PF00501: AMP-binding" amino acids 96 to 492 (397 residues), 149.1 bits, see alignment E=2.5e-47

Best Hits

Predicted SEED Role

"Acetoacetyl-CoA synthetase (EC 6.2.1.16)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Polyhydroxybutyrate metabolism (EC 6.2.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.16

Use Curated BLAST to search for 6.2.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>GFF1990 Acetoacetyl-CoA synthetase (EC 6.2.1.16) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTAPYVPQIRLYQQWLAEHRDLRFESYDALWRWSITDLDGFWQSIWDYFDLRSPTPHTAV
LAKNVMPGAPWFTGARMNYAQQVFRHVGPAHEAGFAAVVSRNEKGRQVELSWPELRRQVA
SLAQHLQAQGVQPGDRVAAYLPNIPEAMVAFLATVSIGAVWSICAPDMGTNAVLDRFRQI
EPVVLIACDGVSYAGRDHDRLAVVAELRAALPTVRHVVLHTNLAEGEAAQAEALRAAAPC
VCFAATTARDDAATAAFEPVWLPFDHPLWIVYSSGTTGLPKPIVHGHGGTVIVGMALKIL
HNDVGCSYHPNSWGERYHWYSSTGWVMWNAQTSGLLNGTTCVIYDGNPGGSKDRPDWTTL
WRFAAELGVTFFGAGAAFFANCIKAGVDLAACGDLSAVRALGTTGSPLSEDAQRWGTEQF
RRVAKSEAQQDIWWCNISGGTDFAGAFIGGNRELPQTPGRMQCRLLGCAVEAWNEQGQPV
IDEVGELVCAQPIPSMPLYFVGDVNHQRLIGSYFDTYPAGQGRKPGGGDLGPEAGAVWRH
GDWLRIGADGSCVIYGRSDATINRHGLRMGTSELYSAVEALPEVMDSMVVDLEYLGRESY
MPLFVVLREGTVLDDALKARINDSIKTALSPRFVPNGVFQVAEIPRTLSGKKQELPIKKL
LLGQPLEKVINREAMANPGCLDWYVKFAASRA