Protein Info for GFF199 in Xanthobacter sp. DMC5

Annotation: Type I secretion system membrane fusion protein PrsE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 447 transmembrane" amino acids 24 to 46 (23 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 24 to 447 (424 residues), 358.9 bits, see alignment E=2e-111 PF00529: CusB_dom_1" amino acids 47 to 402 (356 residues), 34.5 bits, see alignment E=3.1e-12 PF16576: HlyD_D23" amino acids 113 to 367 (255 residues), 30.3 bits, see alignment E=5.1e-11 PF13437: HlyD_3" amino acids 297 to 402 (106 residues), 43.9 bits, see alignment E=7e-15

Best Hits

Swiss-Prot: 34% identical to APRE_PSEAE: Alkaline protease secretion protein AprE (aprE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02022, (no description) (inferred from 69% identity to xau:Xaut_1509)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (447 amino acids)

>GFF199 Type I secretion system membrane fusion protein PrsE (Xanthobacter sp. DMC5)
MTSPAFPDRPFAGASPRPPSGYRSVALLGYALIAVTFLGFGGWAAMARVDGAVVAPGVIG
VESKRQVVQHLEGGIVAAILVQEGQTVRRDETLFRLDPTQSRANDEAALAQRRAALTLEA
RLVAERDDAPAITFPPELLELARVPAVREAMADQIAQFRDRRAAFQSQIDILNGRIVQLG
HELEGLAQERASARQQLAFVEDELTAVRELERKHLVNKSRVSSLEREKARLDGLVGRNIA
DEAKARGSIGEQKMQIVQLTQQRAEDVGSQLLDIREKLAELKEKVQVTRSVLERIEIRAP
SGGIVQNINPRIYTVGAVVRPGDTLLEIVPVDAPLVVEARVPVQDIDRLDLHEAVEVRFP
AFHGRTTPMVLGHLASVSRDRLVDETTHQPYFLARVGVDETDVPADLRSRLQPGMDAEVA
FHTGSRTVMSYLLRPLADAMARAFTER