Protein Info for GFF1987 in Variovorax sp. SCN45

Annotation: Pseudouridine synthase (EC 4.2.1.70), PA2043 type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 306 PF00849: PseudoU_synth_2" amino acids 101 to 249 (149 residues), 100.8 bits, see alignment E=4.4e-33

Best Hits

KEGG orthology group: None (inferred from 87% identity to vpe:Varpa_5379)

Predicted SEED Role

"Similar to ribosomal large subunit pseudouridine synthase A" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (306 amino acids)

>GFF1987 Pseudouridine synthase (EC 4.2.1.70), PA2043 type (Variovorax sp. SCN45)
MTRRRPPKLPLPTRDGVGPSCVGLPYGPWPTIAEFLIERFPAITREAWLERIAANDVIDE
HHVPVTAERRYQSPLRVYYYRSLDNEVHIPFEEQVVFRDDQLLVVDKPPFLPVTPTGKYL
QQSLLVRLKRKLELDDLVPLHRIDRSTSGLVLFSVRPETRGAYQAMFPERRIDKHYEAVV
PWQDGVSSVPATYRSRLVDDEHFMRVREEPGEPNSETHIELLAAQGGHALLKLSPVTGRK
HQLRVHCQALGMPIVNDPIYPVLLPENTDDFDKPLQLLAKSVAFIDPITGEQRRFTSPRS
LSLAPR