Protein Info for GFF1981 in Xanthobacter sp. DMC5

Annotation: Nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 891 PF04879: Molybdop_Fe4S4" amino acids 10 to 58 (49 residues), 58.5 bits, see alignment 1.1e-19 PF00384: Molybdopterin" amino acids 65 to 493 (429 residues), 212.8 bits, see alignment E=1.6e-66 PF01568: Molydop_binding" amino acids 593 to 704 (112 residues), 94.9 bits, see alignment E=6.1e-31 PF04324: Fer2_BFD" amino acids 837 to 884 (48 residues), 54.3 bits, see alignment 2.7e-18

Best Hits

KEGG orthology group: K00372, nitrate reductase catalytic subunit [EC: 1.7.99.4] (inferred from 82% identity to xau:Xaut_2497)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (891 amino acids)

>GFF1981 Nitrate reductase (Xanthobacter sp. DMC5)
MNAPLASPPSVKTTCPYCGVGCGVKATPDGRGGALVEGDPDHPANLGRLCSKGAALDETL
GLETRLLHPLVRGADGVLARASWDDALDRIAGDLRDIIDRHGPQAVAFYLSGQLLTEDYY
AANKLAKGFLGTANVDTNSRLCMSSSVAGHKRGFGSDTVPGSYEDLDRADLIVLTGSNTA
WCHPVLFRRMEAARKARGTKLVVIDPRRTDTGAEADLFLPLAHGTDALLFSGLLVHLAQS
PAFDAAYVEAHTTGFAEALAAARAVAPDAETTARACGLAVEDVERFFALFTKTQRTVTCY
SQGVNQSLVGTDKVNAILNCHLATGRIGREGMGPFSLTGQPNAMGGREVGGLANQLAAHM
GFSPAEVDRVRRFWGAPHIAEREGLKAVDVFAAIGRGEIKALWVMGTNPAVSLPDADGVR
TALEGLELFILSENVQANDTAACRPHVMLPAAAWGEKDGTVTNSERRISRQRPFLPLPGE
VRPDWWALKGVAQRLGFGDAFQWRGPADIFREHAALSGFENDGTRDFDISAFAGLTEAAY
EDITPVQWPLPAGRAEGTARLFAAGGYFTPDRRARFVVPKPVAAASAGGDFPFLLNTGRI
RDQWHTMTRTGLSPRLGAHIPAPFVAVHPDDAAPLGLATGMLAEVSSAHGRAVLEVVVDS
GQQRGTLFAPIHWSHANSSDGRVGALVHAVTDPVSGQPDAKATPAALSPYAAPFEGFVLA
RRPMALPKGVWWARAALEGGYGWRIAGHMGPQDWAQWVTAEGPANVAELSDTPSGLYRAA
AFDADGRLDFAVFIGAAGQRVAWDALKGLLASEVSAADRRLVLSGRRAGANAACGPLVCA
CFGVPKDTILAAIRDGAADAAAVGARLKAGTNCGSCLPEIRKLILEAKAPA