Protein Info for GFF1973 in Xanthobacter sp. DMC5

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 994 PF02739: 5_3_exonuc_N" amino acids 14 to 171 (158 residues), 155.2 bits, see alignment E=2.7e-49 TIGR00593: DNA polymerase I" amino acids 15 to 994 (980 residues), 903.2 bits, see alignment E=1.1e-275 PF01367: 5_3_exonuc" amino acids 195 to 290 (96 residues), 111.1 bits, see alignment E=6e-36 PF01612: DNA_pol_A_exo1" amino acids 385 to 578 (194 residues), 118.9 bits, see alignment E=4.4e-38 PF00476: DNA_pol_A" amino acids 612 to 992 (381 residues), 523.8 bits, see alignment E=5.3e-161

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 81% identity to azc:AZC_4464)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (994 amino acids)

>GFF1973 DNA polymerase I (Xanthobacter sp. DMC5)
MSPSPRPLKPGDHVFLVDGSSFVFRAYFQSINQDRKYNFRSDRLPTGAVRLFCTKLFQFV
RDGAVGIRPTHLAIVFDKSEDSFRKELYPDYKANRSEPPDELIPQFPLMREAVRAFGLIP
VEQARYEADDIIATYAEQAVKAGADVLIVSADKDLMQLIGPKVAMYDPASGESGGRGARP
ERRIGLGEVVEYFGVPPEKVMDVQALAGDSTDNVPGVPGIGIKTAAQLIAEYGDLETLLA
RAGEIKQPKRRESLMNNQDAARISKQLVTLVRDVAVDVPLEDLVLEDPDARRLISFLKAM
EFTTITRRVAEAYGVEAGEVDPDPKLAPSGGDLFAPGGEPAAEAGAKPAQPADATAAPSG
TPTPSDLAHQRAQAARATPVDRKAYRTILDLAELKAWCAKAVDLGVVAFDTETNSLDPMQ
ADLVGVSLALSPNEACYIPLLHQGAGDGLFSEGLLPGQISLADAVAALKPMLEDKGTLKI
GHNVKYDELVLARHGIQVSPIDCTMCMSYALDAGRNGHGMDELSVLHLGHQPIAYSEVTG
KGKAQITFDKVALEPATAYAAEDADVTLRLWQVLKPRLAAEGRTTVYETLERPLIPVLAR
MESRGIAIDKAILARLSSEFAQGAARVEDEIAELAGERLNVGSPKQMGDILFGKMGLPGG
TKTATGMWSTKATALEELAELGHKLPQKILEWRQLSKLRSTYTDALPNFVHPQTKRVHTS
YALAATTTGRLSSSDPNLQNIPVRTEEGRRIRRAFVAEEGNLLVSADYSQIELRLLAEIA
EIPALRQAFTDGLDIHAMTASEMFGVPVKDMPGEIRRRAKAINFGIIYGISAFGLANQLG
IPREEAGQYIKRYFERFPGIRDYMEETKTFCREHGYVETLFGRRCHYPDIAAKNPSLRAF
NERAAINARLQGTAADIIRRAMIRMEPALEKAGLSARMLLQVHDELVFEVPEAEAEATIP
VVRHAMESAAAPAVHLAVPLKVDARAAKNWEEAH