Protein Info for GFF1971 in Xanthobacter sp. DMC5

Annotation: Siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 TIGR01470: siroheme synthase, N-terminal domain" amino acids 28 to 219 (192 residues), 195.3 bits, see alignment E=9.4e-62 PF13241: NAD_binding_7" amino acids 30 to 142 (113 residues), 79.8 bits, see alignment E=2.1e-26 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 248 to 484 (237 residues), 249.9 bits, see alignment E=2.9e-78 PF00590: TP_methylase" amino acids 250 to 461 (212 residues), 168.5 bits, see alignment E=2.1e-53

Best Hits

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 84% identity to xau:Xaut_2375)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (502 amino acids)

>GFF1971 Siroheme synthase (Xanthobacter sp. DMC5)
MAAPGAESSSPRAPRETRQARMEALARLPVFFGLEGRRVLVAGGGEPAAWKAELLSAAGA
RVEVIAPAVCEDIWRLAGAPTHGPIAVHVRDWHPDDIEGAALAIGDFEDAAEAERFSAAV
RAAGVPVNVIDRPALCDFTFGAIVNRSPLVVGISTDGAAPVFGQAVRAKIEALLPSGFRA
WAQAAKTWRAAVTERGLSFHARRAFWERFAAKALSEPHRAPEEADRAALFAAIEIEGTAP
QGRSGAGGSVVLVGAGPGDPELLTLKAVRALQSADVVLYDDLVSGAILDFARREAKKMLV
GKTGYGPSCKQDDINALMVELAREGRRVVRLKGGEPMIFGRAGEEIAHCRAAGVPVEVVP
GISSPQGAASRLVTSLTHRDHARRLQFVTAHARDGKLPADLDFNALSDKAATTVVYMPRR
TLPDLVRNLLAAGIEPATPAVAIFSVTRPEEKVVIATVASLTEAVTRAVDAGAEGPCLVL
YGHALSEVAAQDLPQAAEAARG