Protein Info for GFF1971 in Sphingobium sp. HT1-2

Annotation: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02401: LYTB
  • amino acids 14 to 290 (277 residues), 315.2 bits, E=1.8e-98
• TIGR00216: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • amino acids 14 to 292 (279 residues), 319 bits, E=1.3e-99

Best Hits

Swiss-Prot: 68% identical to ISPH_PHEZH: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) from Phenylobacterium zucineum (strain HLK1)

KEGG orthology group: K03527, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [EC: 1.17.1.2] (inferred from 90% identity to sch:Sphch_1447)

MetaCyc: 54% identical to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Escherichia coli K-12 substr. MG1655)
RXN-24043 [EC: 1.17.7.4]; 1.17.7.4 [EC: 1.17.7.4]

Predicted SEED Role

"4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 1.17.1.2)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
• taxadiene biosynthesis (engineered) (12/13 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• isoprene biosynthesis I (9/10 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.1.2 or 1.17.7.4

Protein Sequence (319 amino acids)

>GFF1971 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4) (Sphingobium sp. HT1-2)
MTQSPAPRPPMTLLIAAPRGFCAGVDRAIIIVERAIEKYGAPVYVRHEIVHNKFVVDSLK
AKGAIFVEELDQVPDGVPVVFSAHGVPKAVPAKAEERGLDYLDATCPLVSKVHRQAERMV
TAGRHILFIGHKGHPEVIGTFGQVPEGTMTLIETVEDAEALAPVDADNLAFLTQTTLSVD
DTAAIVATLERRFPAIIAPKGEDICYATSNRQTAVKAIAARCDAVYVIGAPNSSNSLRLV
EVAEREGTPARLIQRAEEIDFAWLDGVTTLGLTAGASAPEILVREVVDRIAERFAVTEEQ
VETAQENISFKLPRGLEVA