Protein Info for GFF1966 in Variovorax sp. SCN45

Annotation: Apolipoprotein N-acyltransferase / Copper homeostasis protein CutE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 39 to 56 (18 residues), see Phobius details amino acids 68 to 87 (20 residues), see Phobius details amino acids 99 to 124 (26 residues), see Phobius details amino acids 131 to 152 (22 residues), see Phobius details amino acids 172 to 194 (23 residues), see Phobius details amino acids 203 to 220 (18 residues), see Phobius details amino acids 494 to 516 (23 residues), see Phobius details PF20154: LNT_N" amino acids 36 to 190 (155 residues), 91.8 bits, see alignment E=5.5e-30 TIGR00546: apolipoprotein N-acyltransferase" amino acids 73 to 464 (392 residues), 285.8 bits, see alignment E=3.1e-89 PF00795: CN_hydrolase" amino acids 239 to 482 (244 residues), 73.9 bits, see alignment E=1.4e-24

Best Hits

KEGG orthology group: K03820, apolipoprotein N-acyltransferase [EC: 2.3.1.-] (inferred from 84% identity to vpe:Varpa_5419)

Predicted SEED Role

"Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE" in subsystem Phosphate metabolism or Copper homeostasis: copper tolerance (EC 2.3.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>GFF1966 Apolipoprotein N-acyltransferase / Copper homeostasis protein CutE (Variovorax sp. SCN45)
MARAPASPFANLFRLLGFGAAGLAQALSMASPVGGKPLWWLQLLSLGAQVWLLDGLRAQG
AGWRRAGLHGWLFSTAWLTGSFWWLFISMHTYGGLPAPLAAIAVLALAAALGLYYAVAAA
WFVVCAPKGRVTGALVFAALWTLAELVRGSWFTGFPWGAGGYAHVEGPLAALAPWVGVYG
IGAAAALAVALVALHRPLRMSEAVPGVVLLALVFAVPNLVPSLRDDVRGTKGATGKLDVA
LLQGNIPQDEKFIPGGGIETALRWYGEQLRDAKASLVVTPETALPLLPQQLPIGYLDAIQ
ARYSQGNQAAIVGLPMGGPGTYSNSVLGFQPGASQVYQYSKHHLVPFGEFIPMGFRWFIQ
MMNIPLGDFARGGLAQAPFVWQGQRIAPNICYEDLFGDEIGANFRDEASAPTILLNVSNI
AWFGDSVAIDEHLSISRMRALEFARPMVRSTNTGATVVIDAEGRVTHELPRLTRGVLEAS
VEGRRGLTPYARWVAPFGLWPLWILSMAVVAAALVLRGRRRSRD