Protein Info for GFF1951 in Sphingobium sp. HT1-2

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (EC 6.3.2.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 463 PF01225: Mur_ligase" amino acids 24 to 78 (55 residues), 35.5 bits, see alignment 1.6e-12 TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase" amino acids 29 to 453 (425 residues), 362.1 bits, see alignment E=2e-112 PF08245: Mur_ligase_M" amino acids 103 to 295 (193 residues), 153.9 bits, see alignment E=8.2e-49 PF02875: Mur_ligase_C" amino acids 331 to 441 (111 residues), 63.7 bits, see alignment E=4.7e-21

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 85% identity to sjp:SJA_C1-27970)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (463 amino acids)

>GFF1951 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (EC 6.3.2.10) (Sphingobium sp. HT1-2)
LSDLWTSAEIADATGGAVSAPFAVSGVAFDSREVGAGDLFVAMKGETTDGHKFVDKAFAQ
GAAGAIVSEPVDYPHVLVADSAAALEALGRASRKRSNAKIIGVTGSVGKTGTKEALFHAL
ERACPGGVHRSVKSYNNHVGVPLSLARMPRDAAYGVFEMGMNHAGELAALTRQVRPNVAI
VTAIAPAHIEFFGTEEKIAEAKAEIFEGLEPGGTAVIPYDSPHVATLYGKAERHADHILT
FGFSPEADIFVRDTVPAPGGGTLVTAALPGVELCFTVGAPGDHWVSNSLAVLAAVEALGG
DLAAAGLALAEMPGLPGRGARRILPVAGGEALLIDESYNANPLSMAATLKQLGREQADRR
IAVLGGMRELGSRSTELHAALSQPLSENGVDYAILVGEEMAPLADALGGLVAFDHVPDAA
SATDLINKEMRAGDAILVKGSNGIGLSRLVAALGDAARDGDKD