Protein Info for GFF1949 in Sphingobium sp. HT1-2

Annotation: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21799: MurD-like_N
  • amino acids 11 to 94 (84 residues), 35.2 bits, E=1.8e-12
• TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase
  • amino acids 13 to 434 (422 residues), 355.1 bits, E=3.4e-110
• PF08245: Mur_ligase_M
  • amino acids 114 to 239 (126 residues), 79.8 bits, E=4.1e-26
• PF02875: Mur_ligase_C
  • amino acids 297 to 413 (117 residues), 50.7 bits, E=5.2e-17

Best Hits

Swiss-Prot: 64% identical to MURD_SPHAL: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 64% identity to sal:Sala_1883)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Protein Sequence (437 amino acids)

>GFF1949 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9) (Sphingobium sp. HT1-2)
MIVSQAFKGKTYAVLGLARSGLATVETLVASGARVVAWDSREEARAAVADKAELGDPMEI
DLAGFDGIVVSPGVPLNKHPIAMRAKIAGVPIIGDIELFALARPTLPPHKVVGITGTNGK
STTTALIHHIIEQAGFPARMGGNIGLPILGQEPLEPNLHGVGVYVLELSSYQIDLTHSLD
CDVAVLLNITPDHLDRYNGFAGYVASKARLFAMQSADHVAVIATEDEPSRGIAASAPAQL
REVKAADIDPEAQLGWPSLQGPHNAQNAAVAIAVGRALGIDDATIMAALASYASLPHRMQ
AVGTHNGVLYVNDSKATNAASTAPALGAWPARDGKPRIHWILGGVAKSDNLDECAPYYGN
IAHAYTIGEAGHMFADLLRPHMAVDDSEMLSAAVRRAARIAQPGDVVLLSPACASFDQFR
DFEARGDAFAAAVNALE