Protein Info for GFF1931 in Variovorax sp. SCN45

Annotation: RTX toxin transporter, determinant D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 transmembrane" amino acids 76 to 99 (24 residues), see Phobius details TIGR01843: type I secretion membrane fusion protein, HlyD family" amino acids 73 to 494 (422 residues), 355.5 bits, see alignment E=2.1e-110 PF13533: Biotin_lipoyl_2" amino acids 122 to 159 (38 residues), 24 bits, see alignment 6.5e-09 PF13437: HlyD_3" amino acids 345 to 461 (117 residues), 57.6 bits, see alignment E=4.7e-19

Best Hits

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF1931 RTX toxin transporter, determinant D (Variovorax sp. SCN45)
MTPEVSTTAPAANAPHARNMRHPAFELLGRYKAVFKAAWKHRHELAGPRRLSDEAAFLPA
ALSLQDTPVHPAPRRLAYALIALFLIALTWSIFGQIDIVAVAPGKIIVSERTKIIQPLEV
SVVKRVLVRDGDHVEAGQPLVELDPTTANADKTSIDEQLKSVQSEVLRTRALQQALNATT
ASRAPELGKNIPGSWSEVDIDAARAQLSDEWSDITAKLAKASAEINRRQAEIATVREMVA
KLETTVPIARQREADFHQLANQGFMSSHANQDRTRERIELERDLATQRARLAEANATLRE
SENTRAAYIAETKHSLRTREAAAELKRQQGTQDLAKAGQRERLTTLKAPVAGTVQQLAAH
TEGGVVTEAQPLMVIVPDGAQVTAEVTLDNKDIGFVSPMQEAAIKLETFPYTRYGTVNAT
VKTVTADAVNDEKRGAIFPVTLNLNDTTIDVDEKPIKLSPGMNLTAEIKTGKRRVIEFLL
SPVQRASGESMRER